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Progress in Chemistry 2018, Vol. 30 Issue (10): 1464-1474 DOI: 10.7536/PC180528 Previous Articles   Next Articles

Special Issue: 酶化学

• Review •

Rational Design of Artificial Metalloenzymes: Case Studies in Myoglobin

Yingwu Lin1,2*   

  1. 1. School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;
    2. Laboratory of Protein Structure and Function, University of South China, Hengyang 421001, China
  • Received: Revised: Online: Published:
  • Supported by:
    The work was supported by the National Natural Science Foundation of China(No. 31370812,21701081).
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Metalloenzymes play diverse important functions in biological systems. Meanwhile, design of artificial metalloenzymes may fine-tune and expand the functionalities of natural metalloenzymes, and even create novel enzymes with more advanced functions. Myoglobin(Mb) is an ideal protein model for design of heme proteins and other metalloenzymes. In recent years, various approaches have been developed for design of artificial metalloenzymes based on the protein scaffold of Mb, which include design of hydrogen-bonding network, intramolecular disulfide bond, the use of post-translational modifications, introduction of non-natural amino acids and non-native cofactors. This review summaries the recent progress in these aspects, which will help us understand the structure and function relationship of metalloenzymes, master the idea and methodologies of artificial metalloenzyme design, thereby promoting the rapid development in this field.
Contents
1 Introduction
2 Approaches and cases studies
2.1 Design of hydrogen-bonding network
2.2 Design of metal-binding site
2.3 Design of disulfide bond
2.4 Use of post-translational modifications
2.5 Introduction of non-natural amino acids
2.6 Introduction of non-native cofactors
3 Conclusion and outlook
Key words: metalloprotein, metalloenzyme, heme protein, myoglobin, molecular design

CLC Number: 

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