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化学进展 2016, Vol. 28 Issue (1): 58-66 DOI: 10.7536/PC150617 前一篇   后一篇

• 综述与评论 •

用于DNA合成测序的可断裂连接单元研究现状

姜玉1, 谭连江2, 殷燕1, 沈玉梅2*, 龚兵4, 邵志峰3   

  1. 1. 上海应用技术学院化学与环境工程学院 上海 201418;
    2. 上海交通大学系统生物医学研究院 系统生物医学教育部重点实验室 上海 200240;
    3. 上海交通大学生物医学工程学院Bio-ID中心 上海 200240;
    4. 布法罗纽约州立大学化学系 纽约14260 美国
  • 收稿日期:2015-06-01 修回日期:2015-09-01 出版日期:2016-01-15 发布日期:2015-12-21
  • 通讯作者: 沈玉梅 E-mail:yumeishen@sjtu.edu.cn
  • 基金资助:
    国家自然科学基金项目(No.11374207,31370750,81071250,91227109)资助
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Cleavable Linkers in DNA Sequencing by Synthesis

Jiang Yu1, Tan Lianjiang2, Yin Yan1, Shen Yu-Mei2*, Gong Bing4, Shao Zhifeng3   

  1. 1. School of Chemistry and Environmental Engineering, Shanghai Institution of Technology, Shanghai 201418, China;
    2. Shanghai Center for Systems Biomedicine, Key Laboratory of Systems Biomedicine (Ministry of Education), Shanghai Jiao Tong University, Shanghai 200240, China;
    3. Bio-ID Center, School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai 200240, China;
    4. Department of Chemistry, University at Buffalo, State University of New York, Buffalo, NY 14260, United States
  • Received:2015-06-01 Revised:2015-09-01 Online:2016-01-15 Published:2015-12-21
  • Supported by:
    The work was supported by the National Natural Science Foundation of China(No. 11374207, 31370750, 81071250, 91227109).
DNA测序技术是遗传基因组相关疾病研究的基础。合成测序是DNA二代测序技术中非常重要的一种。合成测序技术能够有效地实现大规模平行测序,大大提高测序通量的同时也降低成本,目前已得到广泛的应用。在合成测序中,首先需要合成荧光素标记的核苷酸,作为能够参与DNA链延伸的循环可逆终端。已有文献报道的可逆终端结构主要包括单位点修饰(MRT)和双位点修饰(DRT)两种类型。DRT类型可逆终端的最大优势是DNA聚合酶容易识别且合成路线简单,能够较好地应用于DNA合成测序。在此过程中可断裂连接单元将核苷酸和荧光素连接起来,它的性质直接决定了测序的效率、读长等关键指标。本文主要对目前用于DNA合成测序的可断裂连接单元研究现状进行介绍,并对其发展前景进行了展望。
关键词： DNA合成测序, 循环可逆终端, 可断裂连接单元
DNA sequencing technology is the basis of genetical genomics-related diseases. Sequencing by synthesis is one of the most important second-generation DNA sequencing techniques. Sequencing by synthesis can achieve a massively parallel sequencing effectively and improve sequencing throughput greatly, which favor cost reduction. Therefore, sequencing by synthesis has been widely used over the world. In DNA sequencing by synthesis, fluorescence-labeled nucleotides should be synthesized as a cyclic reversible terminator for DNA extension reaction. The reversible terminators reported in the literature mainly include MRT (mono-modified reversible terminators) and DRT (dual-modified reversible terminators) reversible terminators. The most significant advantage of DRT reversible terminator is that it can be readily identified by DNA polymerase. Besides, the synthetic route of MRT is simple, and this type of reversible terminator is hence more suitable for DNA sequencing by synthesis. Since fluorescence-labeled nucleotides are usually prepared by connecting the fluorescence tag and the nucleotide with a cleavable linker, the properties of the cleavable linker exert significant effects on the key parameters of DNA sequencing such as sequencing efficiency and read length. In this paper, recent advances and current research status of cleavable linkers used in DNA sequencing by synthesis is reviewed. Also, the development prospect of the cleavable linkers is demonstrated.

Contents
1 Introduction
2 Introduction of DNA sequencing technology
3 DNA sequencing by synthesis
3.1 Mono-modified cyclic reversible terminators
3.2 Dual-modified cyclic reversible terminators
4 Linkers
4.1 Enzymatic reversible terminators
4.2 Nucleophilic/alkali sensitive reversible terminators
4.3 Reduction-sensitive reversible terminators
4.4 Photosensitive reversible terminators
4.5 Metal-aided reversible terminators
4.6 Oxidation-sensitive reversible terminators
4.7 Electrophilic/acid-sensitive reversible terminators
5 Outlook

Key words: DNA sequencing by synthesis, cyclic reversible terminator, cleavable linkers

中图分类号: 

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