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化学进展 2006, Vol. 18 Issue (0708): 987-994 前一篇   后一篇

• 综述与评论 •

毛细管电泳无胶筛分介质分离DNA的机理*

周丹;王延梅**   

  1. 中国科学技术大学高分子科学与工程系 合肥 230026
  • 收稿日期:2005-08-01 修回日期:2005-12-01 出版日期:2006-08-24 发布日期:2006-08-24
  • 通讯作者: 王延梅
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Mechanisms of DNA Separation by Capillary Electrophoresis in Non-Gel Sieving Matrices

Dan Zhou;Yanmei Wang**   

  1. Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China

     

  • Received:2005-08-01 Revised:2005-12-01 Online:2006-08-24 Published:2006-08-24
  • Contact: Yanmei Wang
快速、高效而灵敏的分离技术对于DNA的分析是至关重要的。使用无胶筛分介质的毛细管电泳是最重要的DNA分离技术之一，通常使用无交联的高分子溶液作为无胶筛分介质。本文在介绍高分子溶液理论的基础上，综述了DNA在毛细管电泳无胶筛分介质（缠结溶液和稀溶液）中的分离机理，主要包括Ogston筛分模型、各种修正的爬行模型、瞬态缠结偶合机理及其改进机理等。
关键词： 毛细管电泳, DNA分离, 无胶筛分介质, 高分子溶液, 机理
Fast, highly efficient, and sensitive separation technique is crucial for DNA analysis. The capillary electrophoresis using non-gel sieving matrices is one of the most important techniques for DNA separation. The uncrosslinked polymer solutions are generally used as sieving matrices. Based on the introduction of the theory of the polymer solutions, the mechanisms of DNA separation by the capillary electrophoresis in non-gel sieving matrices (entangled and dilute solutions) are reviewed in this paper, which includes Ogston sieving model, various modified reptation models, transient entanglement coupling mechanisms and its improved mechanisms.
Key words: capillary electrophoresis, DNA separation, non-gel sieving matrices, polymer solutions, mechanisms

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