English
新闻公告
More
化学进展 2006, Vol. 18 Issue (0708): 995-1001 前一篇   后一篇

• 综述与评论 •

13C代谢通量分析最新进展

李晓静;陈涛;陈洵;李祥高;赵学明**   

  1. 天津大学化工学院 生物化工系 天津 300072
  • 收稿日期:2005-09-01 修回日期:2006-01-20 出版日期:2006-08-24 发布日期:2006-08-24
  • 通讯作者: 赵学明

Advances in 13C Metabolic Flux Analysis

Xiaojing Li;Tao Chen;Xun Chen;Xianggao Li;Xueming Zhao*   

  1. Deparment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Received:2005-09-01 Revised:2006-01-20 Online:2006-08-24 Published:2006-08-24
  • Contact: Xueming Zhao
代谢通量分析(metabolic flux analysis,MFA)是通过确定代谢网络中代谢流分布来表征细胞代谢状态的强有力的工具。鉴于计量学代谢通量分析在处理复杂代谢网络时表现出的局限性,发展了以13C标记实验为基础的13C MFA。本文介绍了13C MFA的原理与方法,总结和评述了13C MFA在实验与数据分析方面的最新进展以及MFA在功能基因组研究中的重要地位,同时对代谢通量分析的发展前景进行了展望。
Metabolic flux analysis(MFA) is a powerful tool on characterization of cellular state by quantification of intracellular fluxes in metabolic network. For overcoming the limitation of stoichiometric MFA, 13C MFA based on carbon-labeling experiments has been developed. In this paper, the principle and the method of 13C MFA are introduced, the development of 13C MFA in experiment and data analysis are summarized, and the important roles of MFA in the research of functional genomics are reviewed. The future developments of MFA are prospected.

中图分类号: 

()

[ 1 ] 赵学明( Zhao X M) , 白冬梅(Bai D M) 等译. 代谢工程———原理与方法(Metabolic Engineering : Principles and Methodologies) . 北京: 化学工业出版社(Beijing : Chemical Industry Press) , 2003. 2 —3
[ 2 ] Lee S Y, Hong S H , Moon S Y. Genome Informatics , 2002 , 13 :214 —223
[ 3 ] Bai D M, Zhao X M, Li X G, et al . Biotechnol . Bioeng. , 2004 ,88(6) : 681 —689
[ 4 ] Bai D M, Zhao X M, Li X G, et al . Biochem. Eng. J . , 2004 ,18 : 41 —48
[ 5 ] Zamboni N , Fischer E , Muffler A , et al . Biotechnol . Bioeng. ,2005 , 89(2) : 219 —232
[ 6 ] 陈涛(Chen T) , 王靖宇(Wang J Y) , 周士奇(Zhou S Q) 等.化工学报( Journal of Chemical Industry and Engineering) ,2004 , 55(11) : 1842 —1848
[ 7 ] Franzén C J . Yeast , 2003 , 20 : 117 —132
[ 8 ] Dunn W D , Ellis D I. Trends in Anal . Chem. , 2005 , 24 (4) :285 —294
[ 9 ] Wiback S J , Mahadevan R , Palsson B. Biotechnol . Bioeng. ,2004 , 86(3) : 317 —331
[10] Riascos C A M, Gombert A K, Pinto J M. Compu. Chem.Enging. , 2005 , 29 : 447 —458
[11] BlumJ J , Stein R B. Biological Regulation and Development (ed.Goldberger R F) . New York : Plenum Pres , 1982. 99 —124
[12] Fell D A , Small J A. J . Biochem. , 1986 , 238 : 781 —786
[13] Malloy C R , Sherry A D , Jeffrey F M H. J . Biol . Chem. , 1988 ,263 : 6964 —6971
[14] Majewski R A , Domach M M. Biotechnol . Bioeng. , 1990 , 35 :731 —738
[15] Savinell J M, Palsson B a. J . Theor. Biol . , 1992 , 155 : 201 —214
[16] Savinell J M, Palsson B a. J . Theor. Biol . , 1992 , 155 : 215 —242
[17] Katz J , Wals P , Lee W N P. J . Biol . Chem. , 1993 , 268 :25509 —25521
[18] Wiechert W, Siefke C , de Graaf A A , et al . Biotechnol .Bioeng. , 1997 , 55 : 118 —135
[19] Schmidt K, Nielsen J , J . Villadsen J . J . Biotechnol . , 1999 , 71 :175 —185
[20] Sauer U , Bailey J E. Biotechnol . Bioeng. , 1999 , 64 : 750 —754
[21] Schilling C H , Edwards J S , Letscher D , et al . Biotechnol .Bioeng. , 2000 , 71 : 286 —306
[22] Petersen S , de Graaf A A , Eggeling L , et al . J . Biol . Chem. ,2000 , 275 : 35932 —35941
[23] Covert M W, Schilling C H , Palsson B. J . Theor. Biol . , 2001 ,213 : 73 —88
[24] Mahadevan R , Edwards J S , Doyle F J . Biophys. J . , 2002 , 83 :1331 —1340
[25] Beard D A , Liang S C , Qian H. Biophs. J . , 2002 , 83 : 79 —86
[26] Massaoudi M E I , Spelthahn J , Drysch A , et al . Metab. Eng. ,2003 , 5 : 86 —95
[27] Drysch A , Massaoudi M E I. Metab. Eng. , 2003 , 5 : 96 —107
[28] Krêmer J O , Sorgenfrei O , Klopprogge K, et al . J . Bacterio. ,2004 , 186 (6) : 1769 —1784
[29] Wiechert W, Nêh K. Advances in Biochem. Engin. Biotechnol . ,2005 , 92 : 145 —172
[30] Wiechert W, Mê llney M, Petersen S , et al . Meta. Eng. , 2001 ,3 : 265 —283
[31] Dauner M. PhD Thesis of ETH Zürich , Switzerland , 2002
[32] Wiechert W. Metab. Eng. , 2003 , 3 : 195 —206
[33] Wittmann C , Heinzle E. Metab. Eng. , 2001 , 3 : 173 —191
[34] Mollney M, Wiechert W, Kownatzki D , et al . Biotechnol .Bioeng. , 1999 , 66(2) : 86 —103
[35] Szyperski T. Eur. J . Biochem. , 1995 , 232 : 433 —448
[36] Arauzo-Bravo M J , Shimizu K. J . Biotechnol . , 2003 , 105 :117 —123
[37] Fischer E , Zamboni N , Sauer U. Ana. Biochem. , 2004 , 325 :308 —316
[38] Weuster-Botz D. Adv. Biochem. Engin. Biotechnol . , 2005 , 92 :125 —143
[39] Puskeiler R , Kusterer A , John G T , Weuster-Botz D. Biotechnol .Appl . Biochem. , 2005 , 42 :227 —235
[40] Wittmann C , Hyung M, Elmar H. Biotechnol . Bioeng. , 2004 ,87(1) : 1 —6
[41] Balcarcel R R , Clark L M. Biotechnol . Prog. , 2003 , 19 : 98 —108
[42] Sriram G, Shanks J V. Metab. Eng. , 2004 , 6 : 116 —132
[43] Wiechert W, Mê llney M, Isermann N , et al . Biotechnol .Bioeng. , 1999 , 66 : 69 —85
[44] Winden WA , Heijnen J J , Verheijen P J T , et al . Biotechnol .Bioeng. , 2001 , 74(6) : 505 —516
[45] Winden WA , Heijnen J J . Biotechnol . Bioeng. , 2002 , 80 (7) :732 —745
[46] Cornish-Bowden A , Cardenas ML. Nat . Biotechnol . , 2000 , 18 :267 —268
[47] Fernie A R , Geigenberger P , Stitt M. Curr. Opin. Plant Bio. ,2005 , 8 : 1 —9
[48] 赵学明(Zhao X M) , 王靖宇(Wang J Y) , 陈涛(Chen T)等. 生物加工过程( Chinese Journal of Bioprocess Engineering) , 2004 , 2(2) : 1 —7
[49] Huang G S , Hong M Y, Liu Y C. Life Sci . , 2003 , 72 : 2525 —2531
[50] Siddiquee K A , Arauzo-Bravo M J , Shimizu K. Appl .Microbiol .Biotechnol . , 2004 , 63 : 407 —417

[1] 凌盛杰, 邵正中, 陈新. 同步辐射红外光谱成像技术对细胞的研究[J]. 化学进展, 2014, 26(01): 178-192.
阅读次数
全文


摘要

13C代谢通量分析最新进展