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化学进展 2015, Vol. 27 Issue (1): 1-10 DOI: 10.7536/PC140737 前一篇   后一篇

所属专题： 电化学有机合成

• 综述与评论 •

肿瘤标志蛋白的电化学分析

曹亚1, 朱小立1, 赵婧1, 李昊2, 李根喜*1,2   

  1. 1. 上海大学生命科学学院 生物传感技术实验室 上海 200444;
    2. 南京大学生物化学系 医药生物技术国家重点实验室 南京 210093
  • 收稿日期:2014-07-01 修回日期:2014-09-01 出版日期:2015-01-15 发布日期:2014-11-24
  • 通讯作者: 李根喜 E-mail:genxili@nju.edu.cn
  • 基金资助:

    国家杰出青年科学基金项目(No. 20925520)资助

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导出 被引次数 ( 2 | 2 )

Electrochemical Analysis of Tumor Marker Proteins

Cao Ya1, Zhu Xiaoli1, Zhao Jing1, Li Hao2, Li Genxi*1,2   

  1. 1. Laboratory of Biosensing Technology, School of Life Sciences, Shanghai University, Shanghai 200444, China;
    2. State Key Laboratory of Pharmaceutical Biotechnology, Department of Biochemistry, Nanjing University, Nanjing 210093, China
  • Received:2014-07-01 Revised:2014-09-01 Online:2015-01-15 Published:2014-11-24
  • Supported by:

    The work was supported by the National Science Foundation for Distinguished Young Scholars of China (No. 20925520).

随着单克隆抗体技术和免疫学检测技术的不断发展,对癌症相关的肿瘤标志蛋白进行检测分析成为目前癌症早期筛查和诊断最为重要的手段。另一方面,随着分子识别与界面组装技术的发展,电化学检测技术在生物分析领域展现出一些独特的优势,比如操作简单、易于小型化、成本低、灵敏度高等。尤其是近年来,由于特异性结合肿瘤标志蛋白的各种抗体、适体、小分子多肽等被筛选出来,各种纳米材料和纳米技术在电化学分析检测中的应用不断被发掘,新型分子标记技术、界面组装技术以及信号放大技术不断被开发和应用,因此,电化学检测技术在肿瘤标志蛋白的定量分析方面获得了空前的发展机遇,发展极为迅速。本文结合作者所在实验室的一些代表性成果对近年来该领域的研究进展给予简短综述,并对未来的发展前景进行展望。

关键词： 电分析, 生化分析, 生物传感, 蛋白质, 肿瘤标志蛋白

With the development of monoclonal antibody and immunological detection technology, the detection of tumor marker proteins becomes the most important method for early screening and diagnosis of cancer. On the other hand, with the development of molecular recognition and surface assembly techniques, electrochemical analysis displays some unique advantages in biological analysis, such as simple operation, easy-to-miniaturize nature, low cost, sensitivity, and so on. Especially in recent years, various antibodies, aptamers and peptides that can specifically bind with tumor marker proteins have been screened out. Various nanomaterials and nanotechnologies have been explored in the application to electrochemical analysis. Many novel techniques for molecule labeling, surface self-assembly, and signal amplification have been proposed. So, electrochemical analysis obtains unprecedented opportunities in the quantitative detection of tumor marker proteins, and more and more achievements are reported. In this review, by commenting on some typical work conducted in the lab of the authors, we summarize the recent research progress on the electrochemical analysis of tumor marker proteins and make an outlook on the trends of the related research fields in the future.

Contents
1 Introduction
2 Electrochemical immunoassay based on antibody
3 Electrochemical analysis based on aptamer
4 Electrochemical analysis based on polypeptide
5 Electrochemical analysis based on other recognition elements
6 Conclusion and outlook

Key words: electroanalysis, bioanalysis, biosensors, proteins, tumor marker proteins

中图分类号: 

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摘要

肿瘤标志蛋白的电化学分析