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化学进展 2022, Vol. 34 Issue (4): 815-823 DOI: 10.7536/PC210408 前一篇   后一篇

• 综述 •

双光子荧光探针在生物传感中的应用

赵惠1, 胡文博2, 范曲立1,*()   

  1. 1 南京邮电大学 有机电子与信息显示国家重点实验室培育基地 信息材料与纳米技术研究院 南京 210023
    2 西北工业大学 柔性电子研究院 西安 710072
  • 收稿日期:2021-04-08 修回日期:2021-06-08 出版日期:2022-04-24 发布日期:2021-07-29
  • 通讯作者: 范曲立
  • 基金资助:
    江苏省自然科学基金项目(19KJB140014); 江苏省自然科学基金项目(NY219125); 江苏省自然科学基金项目(NY220024)
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Two-Photon Fluorescence Probe in Bio-Sensor

Hui Zhao1, Wenbo Hu2, Quli Fan1()   

  1. 1 State Key Laboratory for Organic Electronics and Information Displays, Institute of Advanced Materials, Nanjing University of Posts and Telecommunications,Nanjing 210023, China
    2 Institute of Flexible Electronics, Northwestern Polytechnical University,Xi’an 710072, China
  • Received:2021-04-08 Revised:2021-06-08 Online:2022-04-24 Published:2021-07-29
  • Contact: Quli Fan
  • Supported by:
    Natural Science Foundation of Jiangsu Province of China(19KJB140014); Natural Science Foundation of Jiangsu Province of China(NY219125); Natural Science Foundation of Jiangsu Province of China(NY220024)

荧光探针由于其灵敏度高、选择性好等优势已经成为研究复杂生物系统的有力工具。和单光子荧光探针相比,双光子荧光探针由于其激发光源较大的穿透深度、低的组织自发荧光干扰以及较好的空间选择性在生物传感中发挥着不可替代的重要作用。在本篇综述中,我们从双光子荧光探针的设计原理出发,系统全面地介绍了双光子荧光探针在金属离子、细胞微环境、活性物质(包含活性氧、活性氮、活性硫)、酶和亚细胞器(线粒体、溶酶体)等检测中的应用研究。在此之后,展望了有机双光子荧光探针在开发和应用方面尚待科学界解决的关键机遇和挑战。

关键词： 荧光探针, 双光子吸收, 生物传感

Fluorescent probes have become a powerful tool for the study of complex biological systems due to their high sensitivity and selectivity. Compared with single-photon fluorescence probe, two-photon fluorescence probe (TPFP) plays an irreplaceable role in biosensor due to its larger penetration depth, lower interference of tissue autofluorescence and better spatial selectivity. In this review, starting from the design strategies of TPFP, we systematically and comprehensively introduce the application of TPFP in the determination of metal ions, celluar microenvironment, reactive species (including reactive oxygen species ROS, reactive nitrogen RNS, reactive sulfur RSS), enzymes, organelles (mitochondria, lysosomes) and so on. After that, we look forward to the key opportunities and challenges in the development and application of organic TPFP.

Contents

1 Introduction

2 Design principle of two-photon fluorescence probes

3 Bio-application of two-photon fluorescence probes

3.1 Two-photon probes for metal ions

3.2 Two-photon probes for cellular microenvironment

3.3 Two-photon probes for reactive species

3.4 Two-photon probes for enzymes

3.5 Two-photon probes for organelles

4 Conclusion and outlook

Key words: fluorescent probe, two-photon absorption, bio-sensor
()
图1 单光子和双光子激发的能量图
Fig. 1 Energy diagram of one-photon and two-photon excitation
图2 光诱导电子转移(PET)(a)、分子内电荷转移(ICT)(b)和荧光共振能量转移(FRET)过程的能量图[8]
Fig. 2 Energy diagram for photoinduced electron transfer (PET) (a), intramolecular charge transfer (ICT) (b) and Förster resonance energy transfer (FRET) process[8]
图3 通过阻断PET过程,用双光子荧光探针检测酸性条件下的Zn2+[11]
Fig. 3 Detection of Zn2+ under acidic conditions with two-photon fluorescence probe, through the blocking of PET processes[11]
图4 用于脑组织和斑马鱼成像的Zn2+响应双光子荧光探针[19]
Fig. 4 Two-photon responses of the Zn2+ probe for imaging in brain tissue and zebrafish[19]. Copyright 2017, American Chemical Society
图5 双光子荧光探针用于比率检测线粒体pH[23]
Fig. 5 Two-photon fluorescence probe for the ratiometric detection of mitochondrial pH[23]
图6 双光子荧光探针用于监测溶酶体极性变化[25]
Fig. 6 Two-photon fluorescence probe for the detection of lysosomal polarity changes[25]
图7 双光子荧光探针用于检测线粒体黏度[26]
Fig. 7 Two-photon fluorescence probe for the detection of lysosomal polarity[26]
图8 基于硫膦基的双光子荧光探针检测 O 2 · -的传感机理[28]
Fig. 8 Sensing mechanism of phosphinothioate-based two-photon fluorescence probe for the detection of O 2 · -[28]. Copyright 2019, American Chemical Society
图9 用于检测H2O2同时释放CO的双光子荧光探针的释放机制[30]
Fig. 9 Two-photon fluorescence probe for the detection of H2O2 and the proposed CO photo-release mechanism[30]
图10 用于检测HClO的双光子荧光探针的检测机制[31]
Fig. 10 The mechanism of two-photon fluorescence probe for the detection of HClO[31]
图11 用于检测NO的双光子荧光探针的检测机制[35]
Fig. 11 The mechanism of two-photon fluorescence probe for the detection of NO[35]
图12 双响应的双光子荧光探针用于检测H2S和HOCl[37]
Fig. 12 Dual-responsive two-photon fluorescent probe for the determination of H2S and HOCl[37]
图13 用于测定BACE1的双光子比率荧光探针的工作原理[39]
Fig. 13 The working principle of the designed two-photon ratiometric fluorescent probe for the determination of BACEl[39]
图14 用于测定线粒体的双光子荧光探针[44]
Fig. 14 The two-photon fluorescent probe for the determination of mitochondria[44]. Copyright 2017, American Chemical Society
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