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化学进展 2015, Vol. 27 Issue (1): 91-102 DOI: 10.7536/PC140738 前一篇   后一篇

• 综述与评论 •

基于SERS探针技术的细胞识别、成像与诊疗

宋春元*, 陈文蔷, 杨琰君, 杨博玥, 苏邵, 汪联辉*   

  1. 南京邮电大学信息材料与纳米技术研究院 有机电子与信息显示国家重点实验室培育基地 江苏省有机电子与信息显示协同创新中心 南京 210023
  • 收稿日期:2014-07-01 修回日期:2014-09-01 出版日期:2015-01-15 发布日期:2014-11-24
  • 通讯作者: 宋春元, 汪联辉 E-mail:iamcysong@njupt.edu.cn;iamlhwang@njupt.edu.cn
  • 基金资助:

    国家重点基础研究发展计划(973)项目(No. 2012CB933301),国家自然科学基金项目(No. 61302027, 21305070, 21475064),江苏省自然科学基金项目(No. BK20130871),“有机与生物光电子学”教育部创新团队项目(IRT1148),生物电子学国家重点实验室开放研究基金(2013G2),江苏省科技支撑计划(No. BE2014719),江苏高校优势学科建设工程(PAPD)和南京邮电大学科研启动基金(NY212032)资助

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Surface-Enhanced Raman Scattering Tags Used in Cell Recognition, Imaging, Diagnosis and Treatment

Song Chunyuan*, Chen Wenqiang, Yang Yanjun, Yang Boyue, Su Shao, Wang Lianhui*   

  1. Key Lab for Organic Electronics & Information Displays (KLOEID), Institute of Advanced Materials (IAM), and Synergetic Innovation Center for Organic Electronics and Information Displays, Nanjing University of Posts & Telecommunications, Nanjing 210023, China
  • Received:2014-07-01 Revised:2014-09-01 Online:2015-01-15 Published:2014-11-24
  • Supported by:

    The work was surpported by the National Basic Research Program of China (973 Program) (No. 2012CB933301), the National Natural Science Foundation of China (No. 61302027, 21305070, 21475064), the Natural Science Foundation of Jiangsu Province (No. BK20130871), the Program for Changjiang Scholars and Innovative Research Team in University (IRT1148), the Open Research Fund of State Key Laboratory of Bioelectronics (2013G2), Sci-tech Support Plan of Jiangsu Province (No. BE2014719), the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD) and Foundation of Nanjing University of Posts and Telecommunications (NY212032).

表面增强拉曼散射(surface-enhanced Raman scattering, SERS),是指吸附在粗糙的金属纳米结构表面的被分析物,在光照射下其拉曼光谱获得显著增强的异常表面光学现象。近年来,SERS技术已广泛地用于物质检测和生物传感等研究,在生物医学领域表现出巨大的应用潜力并取得了令人瞩目的研究成果。本文回顾了SERS探针技术在细胞识别、成像与诊疗等方面的应用及最新研究进展,重点介绍了SERS细胞探针的构建方法与原理,以及基于SERS探针的细胞检测应用策略,并讨论了SERS探针技术在细胞检测中仍有待解决的关键问题。

关键词： 表面增强拉曼散射, SERS探针, 细胞识别, 成像, 诊疗

Surface-enhanced Raman scattering (SERS) refers to an abnormal surface optical phenomenon that Raman spectra of the analyte adsorbed on metal nanostructures can be significantly enhanced under laser irradiation. In recent years, SERS has been widely used in the substance detection and biological sensing, which has achieved significant development and shown great potential applications in the biomedical field. In this paper, the preparation principle and the latest development of SERS tags used in cell detection are introduced, and their recent applications in cell recognition, imaging, diagnosis and treatment are reviewed, as well as the detection strategies and issues related to SERS tag-based cell detection are discussed.

Contents
1 Introduction
2 SERS tags
2.1 Noble metal nanoparticles
2.2 Raman reporter molecules
2.3 Surface coating for protection
2.4 Biofunction with biomolecules
3 Application of SERS tags in cell
3.1 Cell recognition
3.2 Cell imaging
3.3 Cell pH sensing
3.4 Cancer diagnosis and treatment
4 Conclusions and outlooks

Key words: surface-enhanced Raman scattering, SERS tag, cell recognition, imaging, diagnosis and treatment

中图分类号: 

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