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Progress in Chemistry 2009, Vol. 21 Issue (10): 2009-2016 Previous Articles   Next Articles

• Review •

Fabrication of Hydrogenase Molecular Assemblies for BioHydrogen Production

Qian Dongjin**; Liu an   

  1. (Department of Chemistry, Fudan University, Shanghai 200433, China)
  • Received: Revised: Online: Published:
  • Contact: Qian Dongjin E-mail:djqian@fudan.edu.cn
  • Supported by:

    National Natural Science Foundation of China

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Hydrogen is one of the centerpieces of a sustainable, carbon-free energy supply, which has been attracted growing attention because of the rapid consumption of fossil fuel and unacceptable environmental problems such as the greenhouse effect. In nature, some green algae can produce hydrogen after incubation under anaerobic conditions, during which hydrogenase is synthesized and activated. The hydrogenase can reversely catalyze oxidation of hydrogen gas and reduction of protons, thus attracted much attention in the biohydrogen production and biofuel cells. This paper reviews recent developments in the design and assembly of hydrogenase-modified electrodes, wherein the enzyme was immobilized by physical adsorption, self-assembly, sol-gel and Langmuir-Blodgett methods. Electrochemical properties of hydrogenase in the molecular assemblies and biohydrogen production are discussed.

Contents
1 Introduction
2 Physical adsorption
3 Coadsorption
3.1 Polymer-hydrogenase coadsorption
3.2 Clay-hydrogenase-polyviologen coadsorption
3.3 Carbon nanotubes-hydrogenase coadsorption
3.4 Coadsorption of self-assembled monolayers of porphyrin/viologen and hydrogenase
4 Langmuir-Blodgett method
5 Covalently bonding method
6 Sol-gel method
7 Conclusion

CLC Number: 

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