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• Review •

Recent Advances in the Determination of Intracellular Contents in Individual Cells Using Microfluidic Devices

Hao Li1, Xu Chunxiu2, Cheng Heyong2, Liu Jinhua1, Yin Xuefeng1,2   

  1. 1. College of Material, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 310036, China;
    2. Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Hangzhou 310027, China
  • Received: Revised: Online: Published:
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Cells are the fundamental unit of life. Owing to cellular heterogeneity, traditional biochemical assays which analyze cells in bulk often overlook the rich information available when single cells are studied. Accurate and reliable determination of the chemical composition in individual cells would greatly improve the probability of discriminating infected cells from healthy ones and provide a solid foundation on study and development in various fields including biochemistry, medicine, and pathology clinic. Microfluidic chip system for single-cell analysis is now attracting broad interests. The ability of integrating the whole process on one microfluidic chip, including single cell injection, lysis, separation and detection of cellular constituents by chip-based CE, offers a new technical platform for single cell analysis. This article provides a review on recent advances in determining intracellular contents in individual cells using microfluidic devices. Focus areas include on-chip single cell manipulation by means of electroosmotic flow (EOF), pressure-driven flow combined with EOF and optical tweezer (OT) to perform sequentially single cell injection, lysis, separation and detection of cellular constituents by chip-based CE with highly sensitive detection. The approaches on on-chip derivatization are also addressed. Contents 1 Introduction
2 On-chip single cell manipulation by means of electroosmotic flow (EOF)
3 On-chip single cell manipulation by means of pressure-driven flow combined with EOF
4 On-chip single cell manipulation by optical tweezer
5 On-chip cells derivatization
6 Conclusions and outlook
Key words: single-cell analysis, microfluidic chip, on-chip single cell manipulation, determination of intracellular contents

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