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Progress in Chemistry 2012, Vol. 24 Issue (01): 173-181 Previous Articles   Next Articles

• Review •

Carrier Materials of Mesenchymal Stem Cells Expansion

Zhao Shuang1, Zhao Yanyan1, Meng Hengxing2, Li Qian2, Yin Yuji1*   

  1. 1. Tianjin Key Laboratory of Composite and Functional Materials, School of Materials Science and Engineering, Tianjin University, Tianjin 30007;
    2. Union Stem Cell & Gene Engineering CO. LTD., Tianjin 300384, China
  • Received: Revised: Online: Published:
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Mesenchymal stem cell (MSC) is an important cell source of cell therapy and tissue engineering because of its characteristics of self-renewal, multi-differentiation potential, easily isolated and cultured in vitro. Expansion of MSCs in vitro is a necessary step in clinical application of MSCs since it is impossible to get enough MSCs directly from donors. Namely, how to culture MSCs in large-scale is the key factor to limit its application. The methodology of 3-D dynamic culture of anchorage-dependent cells provides an important way for expansion of MSCs in vitro. This review intends to overview the current progress in the MSCs expansion field and discusses the main events that have occurred along the way. Gelatin, alginate, chitosan and some other polysaccharide carrier materials used for 3-D culture of mammalian cells and MSCs are summarized and discussed. The surface modification methodologies of the microcarriers are also presented. Furthermore, some new carrier materials used for stem cells expansion are introduced. The technical advances together with the ever increasing knowledge and experience in the field of carrier materials preparation and MSCs proliferation/expansion characteristics will lead to the realization of the full potential of 3-D dynamic MSCs culture in the future.

Contents
1 Intruduction
2 Conventional carriers of animal cell culture
2.1 Gelatin microcarriers
2.2 Alginate microcarriers
2.3 Chitosan microcarriers
2.4 Other polysaccharide carriers
3 Novel stem cells carriers
4 Prospect

Key words: mesenchymal stem cells (MSCs), expansion, carrier materials, surface modification, 3-D dynamic culture

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