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Progress in Chemistry 2012, Vol. Issue (9): 1785-1793 Previous Articles   Next Articles

• Review •

Enrichment Methods of Phosphopeptides in Proteomics

Li Pengzhang, Wang Yuebo   

  1. School of Chemical Engineering, Shandong University of Technology, Zibo 255049, China
  • Received: Revised: Online: Published:
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The reversible phosphorylation of proteins is recognized as an important branch of proteomics. As one of the most important post-translational modifications, protein phosphorylation plays a very important role in cellular recognition, cellular signal transduction, gene expression and cellular metabolism. Exploration of phosphopeptides using appropriate methods can help us further understand the mechanism of physiology and pathology. However, mass spectra (MS) signals of phosphopeptides are frequently suppressed by the presence of abundant nonphosphopeptides and superabundant of salts which will cause poor selectivity. Therefore, selective enrichment of phosphopeptides is essential to facilitate the MS analysis. This paper summarizes several enrichment techniques of phosphopeptides, including immobilized metal affinity chromatography (IMAC), metal oxide enrichment methods, strong cation exchange chromatography (SCX), strong anion exchange chromatography (SAX) and matrix assisted laser desorption ionization (MALDI) on-plate enrichment methods, especially, the materials used in these enrichment methods have been discussed in detail. At the end of this review, the advantages and disadvantages of each method are summarized, and a prospect of effective enrichment strategies of phosphopeptides is given. Contents 1 Introduction
2 Enrichment methods of phosphopeptides
2.1 Immobilized metal affinity chromatography
2.2 Metal oxide enrichment methods
2.3 Strong anion and cation exchange chromatography
2.4 Phosphopeptide enrichment on MALDI plate
3 Conclusion and outlook
Key words: phosphopeptides, immobilized metal affinity chromatography (IMAC), metal oxide enrichment methods, strong cation exchange chromatography (SCX), strong anion exchange chromatography (SAX), MALDI on-plate enrichment methods

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