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Progress in Chemistry 2010, Vol. 22 Issue (11): 2215-2223 Previous Articles   Next Articles

• Review •

Recent Advances in Single-Cell Analysis Using Capillary Electrophoresis

Deng Bin   Xu Jianrong   Wang Zhiming   Xiao Yuxiu **   

  1. (College of Pharmacy, Wuhan University, Wuhan 430071, China)
  • Received: Revised: Online: Published:
  • Contact: Xiao Yuxiu E-mail:yuxiuxiao@yahoo.com.cn
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Cells are the fundamental building blocks of life. Single-cell analysis can explain structures and functions of individual cells and uncovers the heterogeneity within populations of cells. It plays an important role in researches of cellular differentiation, physiology, pathology and early clinic diagnosis. As a high efficient separation method, capillary electrophoresis is uniquely suited for single-cell analysis because it needs ultrasmall samples and can be coupled  with sensitive detectors. The recent advances since 2007 in single-cell analysis using capillary electrophoresis are described, including single-cell injection techniques, single-cell lysis techniques, detection methods and applications. Future directions of single-cell analysis by capillary electrophoresis are also predicted. It is shown that capillary electrophoresis is not only suited for single-cell analysis but also still has tremendous development potentials and wide application fields.

Contents
1 Introduction
2 Single-cell injection techniques
3 Single-cell lysis techniques
4 Detection methods
4.1 Optical absorption-based detection
4.2 Laser-induced fluorescence
4.3 Chemiluminescence and electrochemiluminescence
4.4 Electrochemical detection
4.5 MS
5 Applications
5.1 Proteins and proteome
5.2 Metabolome
5.3 Subcellular structures
5.4 Neurotransmitters
5.5 Microorganisms
5.6 Others
6 Conclusions and outlook

Key words: capillary electrophoresis, single-cell analysis, single-cell injection, detection, applications

CLC Number: 

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