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Progress in Chemistry 2010, Vol. 22 Issue (11): 2199-2206 Previous Articles   Next Articles

• Review •

Quantification of Proteins by Use of ICP-MS

Zheng Lingna1,2  Wang Meng2**   Wang Huajian2   Li Jianjun1  Feng Weiyue2   Chai Zhifang2   

  1. (1. Department of Chemistry, Zhengzhou University, Zhengzhou 450001,China; 2. Key Laboratory of Nuclear Analytical Techniques and Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety, Institute of High Energy Physics, Chinese Academy of Sciences, Beijing 100049,China)
  • Received: Revised: Online: Published:
  • Contact: Wang meng E-mail:wangmeng@ihep.ac.cn
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Proteomics has become one of the most active fields in life sciences. The study of biological functions of proteins not only relies on high-throughput identification of proteins, but also on quantitative analysis of the dynamic proteins that is termed quantitative proteomics. Quantitative proteomics is anticipated to provide new insights into biological functions, facilitate the identification of prognostic disease markers and contribute to discovery of proteins as therapeutic targets. The available methods for quantitative proteomics are mainly based on the isotope tagging combined with biological mass spectrometry (such as Electrospray Ionization Mass Spectrometry, ESI-MS, and Matrix Assisted Laser Desorption Ionization Mass Spectrometry, MALDI-MS). Recently inductivity coupled plasma-mass spectrometry (ICP-MS), as an attractive complement to ESI-MS and MALDI-MS, has played an increasing role in protein quantification, especially in absolute protein quantification. ICP-MS is an ideal instrument for determination of trace elements in biomolecules because of its unique advantages, such as high sensitivity, wide dynamic range, and minimal matrix effects. This review will selectively discuss the recent advances of ICP-MS based techniques and their applications in protein quantification and immunoassay.

Contents 
1 Introduction
2 Problems of protein quantification 
3 Available methods for protein quantification 
4 The advantages of ICP-MS in proteins quantification 
5 ICP-MS-based methods for protein quantification
5.1 Protein quantification based on element labeling and ICP-MS detection
5.2 ICP-MS-based Immunoassay
6 Conclusions and outlook

Key words: ICP-MS, ESI-MS, MALDI-MS, proteins quantification, element labeling, immunoassay

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