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Progress in Chemistry 2010, Vol. 22 Issue (09): 1826-1835 Previous Articles   Next Articles

• Review •

Biomaterials for Spheroidal Aggregate Culture of Hepatocytes

Qiu Yuan1,2  Zhang Jichuan2   Gao Changyou2,3**   

  1. (1.Zhejiang-California International NanoSystems Institute, Hangzhou 310012, China;2. Key Laboratory of Macromolecular Synthesis and Functionalization of Ministry of Education, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China ;3.State Key Laboratory for Diagnosis and Treatment of Infectious Disease,Zhejiang University, Hangzhou 310003, China )
  • Received: Revised: Online: Published:
  • Contact: Gao Changyou E-mail:cygao@zju.edu.cn;cygao@mail.hz.zj.cn
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In the study of therapies for liver diseases, the in vitro culture of hepatocytes is one of the key issues since these cells are apt to lose their normal phenotype and liver-specific functions as a result of the change of culture microenvironment in vitro. It has been shown that the hepatocyte spheroidal aggregates can better maintain the normal structures and functions of hepatocytes, and thereby are more attractive for therapeutic applications. In this article, the recent progress on hepatocyte spheroidal aggregates is reviewed. It starts with the introduction of the status of hepatocytes in sinusoids and cellular architecture of the liver , following with detail discussion on influences of biomaterials on the hepatocyte spheroidal aggregates in terms of morphology and functions. These factors include chemical composition like galactose and RGD, charging property, hydrophilicity/hydrophobicity, surface topology, and three-dimensional scaffolds. Other factors such as culture environment, addition of bioactive factors, and co-culture with other types of cells are also introduced briefly. Finally, perspectives of study on the spheroidal aggregates are suggested.

Contents 
1 Introduction
2 Influences of biomaterials on hepatocyte spheroidal aggregates
2.1 Culture substrates modified with galactose
2.2 Multifunctional substrates modified with galactose
2.3 charged substrates
2.4 Hydrophilicity/hydrophobicity of the substrates
2.5 Topographical substrates
2.5 Three-dimensional scaffolds
3 Other factors beyond the biomaterials
4 Conclusions and Perspectives

Key words: hepatocyte, spheroidal aggregates, in vitro, biomaterials

CLC Number: 

［1 ］ Strain A J，Neuberger J M. Science，2002，295: 1005—1009
［2 ］ Lee W M N. Engl. J. Med. ，1993，329: 1862—1872
［3 ］ Allen J W，Hassanein T，Bhatia S N. Hepatology，2001，34:
447—455
［4 ］ Patzer J F，Ann N Y. Acad. Sci. ，2001，944: 320—333
［5 ］ Carpentier B，Gautier A，Legallais C. Gut，2009，58: 1690—
1702
［6 ］ Berthiaume F，Moghe P V，Toner M，et al. Faseb. J. ，1996，
10: 1471—1484
［7 ］ Landry J，Bernier D，Ouellet C，et al. J. Cell. Biol. ，1985，
101: 914—923
［8 ］ Brophy C M， Luebke-Wheeler J L， Amiot B P， et al.
Hepatology，2009，49: 578—586［9 ］ Hansen L K，Hsiao C C，Friend J R，et al. Tissue Eng. ，1998，
4: 65—74
［10］ Hodgkinson C P，Wright M C，Paine A J. Mol. Pharmacol. ，
2000，58: 976—981
［11］ Kim S H，Kim J H，Akaike T. Febs Lett. ，2003，553: 433—
439
［12］ Chung T W，Yang J，Akaike T，et al. Biomaterials，2002，23:
2827—2834
［13］ Park I K，Yang J，Jeong H J，et al. Biomaterials，2003，24:
2331—2337
［14］ Yang J，Goto M，Ise H，et al. Biomaterials，2002，23: 471—
479
［15］ Gotoh Y，Niimi S，Hayakawa T，et al. Biomaterials，2004，25:
1131—1140
［16］ Tobe S，Takei Y，Kobayashi K，et al. Biochem. Biophys. Res.
Commun. ，1992，184: 225—230
［17］ Yoon J J，Nam Y S，Kim J H，et al. Biotechnol. Bioeng. ，
2002，78: 1—10
［18］ Tan H P，Lao L H，Wu J D，et al. Polym. Adv. Technol. ，
2008，19: 15—23
［19］ Ying L，Yin C，Zhuo R X，et al. Biomacromolecules，2003，4:
157—165
［20］ Seo S J，Akaike T，Choi Y J，et al. Biomaterials，2005，26:
3607—3615
［21］ Seo S J，Park I K，Yoo M K，et al. J. Biomat. Sci-Polym. E，
2004，15: 1375—1387
［22］ Kim S H，Hoshiba T， Akaike T. Biomaterials，2004，25:
1813—1823
［23］ Kim S H，Hoshiba T，Akaike T. J. Biomed. Mater. Res. Part
A，2003，67A: 1351—1359
［24］ Glicklis R，Merchuk J C，Cohen S. Biotechnol. Bioeng. ，2004，
86: 672—680
［25］ Harris A L. Nat. Rev. ，Cancer，2002，2: 38—47
［26］ Du Y N，Chia S M，Han R B，et al. Biomaterials，2006，27:
5669—5680
［27］ Du Y，Han R B，Wen F，et al. Biomaterials，2008，29: 290—301
［28］ Chew S Y，Mi R F，Hoke A，et al. Adv. Funct. Mater. ，2007，
17: 1288—1296
［29］ Yim E K F，Wen J，Leong K W. Acta Biomater. ，2006，2:
365—376
［30］ Chew S Y，Wen J，Yim E K F，et al. Biomacromolecules，
2005，6: 2017—2024
［31］ Chew S Y，Hufnagel T C，Lim C T，et al. Nanotechnology，
2006，17: 3880—3891
［32］ Chua K N，Lim W S，Zhang P C，et al. Biomaterials，2005，
26: 2537—2547
［33］ Feng Z Q，Chu X H，Huang N P，et al. Biomaterials，2009，
30: 2753—2763
［34］ Koide N， Sakaguchi K，Koide Y， et al. Exp. Cell. Res. ，
1990，186: 227—235
［35］ Ijima H，Matsushita T，Nakazawa K，et al. Tissue Eng. ，1998，
4: 213—226
［36］ Matsushita T，Nakano K，Nishikura Y，et al. Cytotechnology，
2003，42: 57—66
［37］ Kidambi S，Lee I，Chan C. J. Am. Chem. Soc. ，2004，126:
16286—16287
［38］ Janorkar A V， Rajagopalan P， Yarmush M L， et al.
Biomaterials，2008，29: 625—632
［39］ Ma Z W，Mao Z W，Gao C Y. Colloid Surface B，2007，60:
137—157
［40］ 彭承宏( Peng C H) ，韩宝三( Han B S) ，高长有( Gao C Y)
等. 中华外科杂志( Chinese Journal of Surgery ) ，2004，42
(17) : 1064—1068
［41］ 彭承宏( Peng C H) ，韩宝三( Han B S) ，高长有( Gao C Y)
等. 中华医学杂志(National Medical Journal of China) ，2004，
84(17) : 1460—1464
［42］ Wittmer C R，Phelps J A，Lepus C M，Saltzman W M，Harding
M J，Van Tassel P R. Biomaterials，2008，29: 4082—4090
［43］ Tsai W B，Lin J H. Acta Biomater. ，2009，5: 1442—1454
［44］ Flemming R G，Murphy C J，Abrams G A，et al. Biomaterials，
1999，20: 573—588
［45］ Krasteva N，Seifert B，Albrecht W，et al. Biomaterials，2004，
25: 2467—2476
［46］ Smith L A，Liu X H，Hu J，et al. Biomaterials，2009，30:
2516—2522
［47］ Fukuda J，Nakazawa K. Tissue Eng. ，2005，11: 1254—1262
［48］ Huang H，Oizumi S，Kojima N，et al. Biomaterials，2007，28:
3815—3823
［49］ Lee J，Cuddihy M J，Cater G M，et al. Biomaterials，2009，30:
4687—4694
［50］ Bhatia S N，Balis U J，Yarmush M L，et al. Faseb J. ，1999，
13: 1883—1900
［51］ Thomas R J，Bennett A，Thomson B，et al. Eur. Cells Mater. ，
2006，11: 16—26
［52］ Kidambi S， Sheng L F， Yarmush M L， et al. Macromol.
Biosci. ，2007，7: 344—353
［53］ Hannachi I E， Itoga K， Kumashiro Y， et al. Biomaterials，
2009，30: 5427—5432
［54］ Miyazawa M，Torii T，Toshimitsu Y，et al. J. Gastroenterol.
Hepatol. ，2007，22: 1959—1964
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