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Progress in Chemistry 2010, Vol. 22 Issue (08): 1648-1655 Previous Articles   Next Articles

• Review •

Surface Plasmon Resonance Imaging

Shen Gangyi1,2  Chen Yi1**   Zhang Yiming1  Cui Jian2   

  1. (1. Key Laboratory of  Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China; 2. The Key Laboratory of Chinese Minority Traditional Medicine, Ministry of Education of China, Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China)
  • Received: Revised: Online: Published:
  • Contact: Chen Yi E-mail:chenyi@iccas.ac.cn
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This review introduces a novel microdot sensing method, surface plasmon resonance imaging( SPRI ), which is a label-free method suitable for in situ and real time monitoring of molecular interaction events simultaneously. It is becoming a new and promising platform for high-throughput study of a huge amount of biological samples with limited volume or resources. In addition to the introduction of its working principle, method development, and applications to the characterization of thin film and biomolecular interaction monitoring, the state-of-art of this method is particularly focused and discussed to have an insight into its future development trends.

Contents 
1 Introduction 
2 Principle and history of SPRI 
3 Applications 
3.1 Film characterization 
3.2 Study of biomolecular interactions 
4 Technological development 
4.1 Instrumental advancement 
4.2 Signal amplification 
4.3 Coupling of SPRI with other methods
4.4 New technique for preparation and functionalization of gold sensing film 
5 Outlook

CLC Number: 

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Abstract

Surface Plasmon Resonance Imaging