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Progress in Chemistry 2010, Vol. 22 Issue (04): 706-712 Previous Articles   Next Articles

• Invited Article •

Characterization of Polymers by High Performance Liquid Chromatography

Zhong Yalan1; Jiang Xulin1,2,**   

  1. （1. College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China; 2. Key Laboratory of Biomedical Polymers of Ministry of Education, Wuhan University, Wuhan 430072, China)
  • Received: Revised: Online: Published:
  • Contact: Jiang Xulin E-mail:xljiang@whu.edu.cn
  • Supported by:

    National Natural Science Foundation of China

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Size-exclusion chromatography (SEC) is the most commonly applied high performance liquid chromatography (HPLC) technique separating molecules based on the size for the measurement of the molecular-weight averages and the molecular-weight distribution (MWD) for polymers. Interactive HPLC, which is based on molecular interactions between the polymer molecules and the mobile and stationary phases in the column, can be used to separate polymers based on chemical composition or functionality (functional groups or end-groups). Interactive LC is superior when compared to SEC, with respect to selectivity and peak capacity. In this review, the different HPLC modes are introduced for separation and characterization of polymers. The progress on SEC, interactive HPLC, critical liquid chromatography and comprehensive two dimensional LC of polymers is summarized. The limitations and prospective applications of the technique for separation and characterization of polymers are also discussed.

Contents
1 Introduction
2 Separation modes in liquid chromatography of polymers
3 Size-exclusion chromatography of polymers
4 Interactive liquid chromatography of polymers
5 Critical liquid chromatography of polymers
6 Comprehensive two dimensional liquid chromatography of polymers
7 Outlook

Key words: high performance liquid chromatography(HPLC), macromolecules, separation and characterization, molecular-weight distribution, chemical-composition distribution

CLC Number: 

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