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Progress in Chemistry 2010, Vol. 22 Issue (04): 593-602 Previous Articles   Next Articles

• Invited Article •

Magnetic Iron Oxide Nanoparticles Immobilized Enzymes

Xin Baojuan; Xing Guowen**   

  1. (College of Chemistry, Beijing Normal University, Beijing 100875, China)
  • Received: Revised: Online: Published:
  • Contact: Xing Guowen E-mail:gwxing@bnu.edu.cn
  • Supported by:

    National Natural Science Foundation of China

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Nanoparticles can be used as the carriers of immobilized enzymes，and the magnetic nanoparticle immobilized enzymes are easy to operate, separate and recover from the reaction system. Moreover, utilizing an external magnetic field the movement manner and direction of the immobilized enzyme can be controlled to improve their catalytic efficiency, compared with the traditional mechanical stirring ways. Among many nano-materials, iron oxide has received considerable attention because of its magnetic, catalytic, and other good characteristics of high-profile. In this paper, a variety of magnetic iron oxide nanoparticles immobilized enzymes, especially the preparation and application of immobilized lipase and protease are reviewed. The advantages and shortcomings of the magnetic iron oxide nanoparticles immobilized enzymes and the future developing prospects are also discussed.

Contents
1 Introduction
2 Magnetic iron oxide nanoparticles immobilized lipase
2.1 Magnetic polymer microsphere immobilized lipase
2.2 Magnetic nanoparticles directly immobilized lipase
2.3 Magnetic siliceous mesocellular foam immobilized lipase
3 Magnetic iron oxide nanoparticles immobilized protease
3.1 Immobilized α-chymotrypsin
3.2 Immobilized papain
3.3 Immobilized trypsin
4 Other magnetic iron oxide nanoparticles immobilized enzymes
5 Conclusion and outlook

Key words: iron oxide, nanoparticles, immobilized enzymes

CLC Number: 

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