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Progress in Chemistry 2010, Vol. 22 Issue (01): 201-209 Previous Articles   Next Articles

• Invited Article •

Metal Complexes and Their Applications in Bioassay, Bio-Imaging and Protein Staining

Zhou Ming*;  Fei Hao;  Liu Yang;  Li Wanfei   

  1. (Suzhou Institute of Nano-Tech and Nano-Bionics, Chinese Academy of Sciences, Suzhou 215125, China)
  • Received: Revised: Online: Published:
  • Contact: Ming Zhou E-mail:mzhou2007@sinano.ac.cn
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Metal complexes, which possess unique photophysical and photochemical properties, have played important roles in many fields of life sciences and medical diagnostics. The rapidly expanding family of metal complexes, with large Stokes-shifts, multi-color tunability and stability against photobleaching, represents an attractive resource to chemists and life scientists seeking novel bio-labeling and bio-imaging methodologies. In this mini-review, we describe several well established bioanalytical and in vitro diagnostic systems that utilize ruthenium and rare earth metal complexes as the luminescent labeling molecules, gadolinium complexes as contrast agents in magnetic resonance imaging and novel iridium complexes as cell staining or protein staining agents. In addition, the construction of nano-architecture and combination with nanotechnology for biomedical application of potential metal complexes are also briefly introduced.

Contents
1 Introduction
2 Metal complexes used in commercial technologies
2.1 Ru(bpy)32+ and other ruthenium complexes containing N^N bidentate ligands
2.2 Luminescent rare earth metal complexes
2.3 Contrast agents for magnetic resonance imaging
3 Iridium complexes and their potential applications
3.1 Cellular imaging
3.2 Bio-labeling
3.3 Protein assay
4 Metal complex nano-architectures for bioassay
5 Summary and prospects

Key words: metal complexes, bioassays, bio-labeling, protein staining, bio-imaging

CLC Number: 

[ 1 ] 　Bradford M M. Anal. Biochem. , 1976, 72: 248—254
[ 2 ] 　Medintz IL, Uyeda H T, Goldman E R, Mattoussi H. Nature Materials, 2005, 4: 435—446
[ 3 ] 　Singer V L, Lawlor T E, Yue S. Mutation Research, 1999,439: 37—47
[ 4 ] 　Zipper H, Brunner H, Bernhagen J, Vitzthum F. Nucleic Acids Res. , 2004, 32: e103
[ 5 ] 　Tanaka K, Tainaka K, Kamei T, Okamoto A. J. Am. Chem.Soc. , 2007, 129: 5612—5620
[ 6 ] 　RichterM M. Chem. Rev. , 2004, 104: 3003—3036
[ 7 ] 　MiaoW. Chem. Rev. , 2008, 108: 2506—2553
[ 8 ] 　DeaverD R. Nature, 1995, 377: 758—760
[ 9 ] 　Yang H, Leland J K, Yost D, Massey R J. Nature Biotech. ,1994, 12: 193—194
[ 10 ] 　Blackburn G F, Shah H P, Kenten J H, Leland J, Kamin R A,Link J, Peterman J, PowellM J, Shah A, Talley D B, Tyagi SK, Wilkins E, Wu T G, Massey R. J. Clin. Chem. , 1991, 37:1534—1539
[ 11 ] 　Kenten J H, Casadei J, Link J, Lupold S, Willey J, Powell M,ReesA, Massey R. J. Clin. Chem. , 1991, 37: 1626—1632
[ 12 ] 　ZhouM, Robertson G P, Roovers J. Inorg. Chem. , 2005, 44:8317—8325
[ 13 ] 　MSD Technology Brochure, 2006Meso Scale Diagnostics. [ 2009-01-05 ]. http://www.mesoscale.com/catalogsystemweb/webroot/literature/brochures/pdf/techBrochure.pdf
[ 14 ] 　Lu Y, WongW L, Meng Y G. J. Immunol. Methods, 2006,314: 74—79
[ 15 ] 　Lanne B, Panfilov O. J. Proteome Res. , 2005, 4: 175—179
[ 16 ] 　Berger K, Wissmann D, Ihling C, Kalkhof S, Beck-Sickinger A,SinzA, Paschke R, Führer D. Mol. Cell Endocrinol. , 2004,227: 21—32
[ 17 ] 　Lamanda A, Zahn A, Roder D, Langen H. Proteomics, 2004,4: 599—608
[ 18 ] 　Yuan J, Wang G J. Fluoresc. , 2005, 15: 559—568
[ 19 ] 　Hemmila I, Laitala V. J. Fluoresc. , 2005, 15: 529—542
[ 20 ] 　Caravan P, Ellison J J, McMurry T J, Lauffer R B. Chem.Rev. , 1999, 99: 2293—2352
[ 21 ] 　俞开潮(Yu K C) , 王国平(Wang G P) , 丁尚武(Ding SW) ,叶朝辉( Ye C H) . 波谱学杂志(Chinese Journal of Magnetic Resonance) , 2004, 21: 505—525
[ 22 ] 　冯志明( Feng ZM) , 马俊(Ma J ) . 国际生物医学工程杂志( International Journal of Biomedical Engineering) , 2006, 29:231—234
[ 23 ] 　Highly Efficient OLEDs with Phosphorescent Materials ( Ed.Yersin H) . Weinheim Wiley-VCH, 2008
[ 24 ] 　ZhongW, Urayama P, MycekM A. J. Phys. D: App l. Phys. ,2003, 36: 1689—1695
[ 25 ] 　[ 2008-12 ]. http://www.rubipy.com; http: //www.sunatech.com
[ 26 ] 　Amoroso A J, Coogan M P, Dunne J E, Fernández-Moreira V,Hess J B, HayesA J, Lloyd D, Millet C, Pope S J A, Williams C. Chem. Commun. , 2007, 3066—3068
[ 27 ] 　Yu M, Zhao Q, ShiL, Li F, Zhou Z, Yang H, Yi T, Huang C.Chem. Commun. , 2008, 18: 2115—2117
[ 28 ] 　Lo K KW, Lee P K, Lau J S Y. Organometallics, 2008, 27:2998—3006
[ 29 ] 　Lo K KW, Lee T KM, Lau J S Y, PoonW L, Cheng S H. Inorg. Chem. , 2008, 47: 200—208
[ 30 ] 　Lo K KW, NgD CM, Chung C K. Organometallics, 2001, 20:4999—5001
[ 31 ] 　Lo K KW, Chung C K, Lee T KM, Lui L H, Tsang K H K,Zhu N. Inorg. Chem. , 2003, 42: 6886—6897
[ 32 ] 　Lo K KW, HuiW K, Chung C K, Tsang K H K, Ng D C M,Zhu N, Cheung K K. Coord. Chem. Rev. , 2005, 249: 1434—1450
[ 33 ] 　李宛飞(LiW F) , 周明( Zhou M) , 刘扬(Liu Y) , 黄泽柱(Huang Z Z) , 王鑫(Wang X) . CN 200910208649. 1, 2009
[ 34 ] 　MellorD P, Maley L E. Nature, 1950, 165: 453—454
[ 35 ] 　Ma D L, WongW L, ChungW H, Chan F Y, So P K, Lai T S,Zhou Z Y, Leung Y C, Wong K Y. Angew. Chem. Int. Ed. ,2008, 47: 3735—3739
[ 36 ] 　Research Highlight, Nature Asia Materials, ( 2008-6-30 ) .[ 2009-01-05 ]. http://www.natureasia.com/asia-materials/highlight.php? id = 137
[ 37 ] 　Zhou M, Roovers J. Macromolecules, 2001, 34 : 244—252
[ 38 ] 　Zhou M, Roovers J, Robertson G P, Grover C P. Anal. Chem. ,2003, 75: 6708—6717
[ 39 ] 　Lian W, Litherland S A, Badrane H, Tan W, Wu D, Baker H V, Gulig P A, Lim D V, J in S. Anal. Biochem. , 2004, 334:135—144
[ 40 ] 　Zhou X, Xing D, Zhu D, J ia L. Anal. Chem. , 2009, 81:255—261
[ 41 ] 　Trau D, YangW, Seydack M, Caruso F, Yu N T, Renneberg R. Anal. Chem. , 2002, 74: 5480—5486
[ 42 ] 　Wang J, Polsky R, Turner K L. et al. US 7176036, 2007
[ 43 ] 　LiM J, Chen Z F, Yam V W W, Zu Y B. ACS NANO, 2008,2: 905—912
[ 44 ] 　Zhang L H, Dong S J. Anal. Chem. , 2006, 78: 5119—5123
[ 45 ] 　Zhang L H, Dong S J. Electrochem. Commun. , 2006, 8:1687—1691
[ 46 ] 　Zhang L H, Liu B F, Dong S J. J. Phys. Chem. B, 2007,111: 10448—10452
[ 47 ] 　Huo S, Deaton J C, Rajeswaran M, Lenhar W C. Inorg.Chem. , 2006, 45: 3155—3157
[ 48 ] 　McGee K A, Mann K A. Inorg. Chem. , 2007, 46:7800—7809
[ 49 ] 　McDonald A R, Lutz M, von Chrzanowski L S, van Klink G P M, Spek A L, van Koten G. Inorg. Chem. , 2008, 47: 6681—6691
[ 50 ] 　Blackman M L, Royzen M, Fox J M. J. Am. Chem. Soc. ,2008, 130: 13518—13519
[ 51 ] 　Devaraj N K, Weissleder R, Hilderbrand S A. Bioconjugate Chem. , 2008, 19: 2297—2299
[ 52 ] 　费浩( Fei H) , 周明( Zhou M) , 贾俊丽( J ia J L ) , 王小波(Wang X B ) , 黄泽柱( Huang Z Z) . CN 200810242798.5, 2008
[ 53 ] 　Hermanson G T. Bioconjugate Techniques, 2nd ed. Academic Press, 2008
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