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Progress in Chemistry 2010, Vol. 22 Issue (01): 125-132 Previous Articles   Next Articles

• Invited Article •

Smart Polymers Based Aqueous Two-Phase Systems Applied in Bio-molecules Separation and Purification

Qu Feng**;  Lv Fenghua;  Zhang Huijuan   

  1. ( School of Life Science & Technology, Beijing Institute of Technology, Beijing 100081, China)
  • Received: Revised: Online: Published:
  • Contact: Qu Feng E-mail:qufengqu@bit.edu.cn
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Smart polymer based aqueous two-phase systems (ATPS) exhibit fast separation of target molecules with polymers and convenient recycle of polymers by simply changing environmental conditions such as temperature, pH, ionic strength and light or radiation forces. In recent years, the application of smart polymer based aqueous two-phase systems in separation and purification of bio-molecules is developing fast. In this paper, the principle of ATPS is introduced briefly. The applications of smart polymer based aqueous two-phase systems such as temperature-sensitive, pH-sensitive, photo-response, affinity functional, and combination of aqueous two-phase systems with related techniques in bio-molecule separation and purification in more than ten years are reviewed.

Contents
1 Introduction
2 The principle and the theoretical model of aqueous two-phase extraction
3 Studied of smart polymers based aqueous two-phase systems
3.1 Temperature-sensitive aqueous two-phase systems
3.2 pH-sensitive aqueous two-phase systems
3.3 Photo-response aqueous two-phase systems
3.4 Affinity aqueous two-phase systems
3.5 Combination of aqueous two-phase systems with related techniques
4 Prospects

Key words: smart polymers, aqueous two-phase systems, bio-molecules, separation and purification

CLC Number: 

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