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Progress in Chemistry 2009, Vol. 21 Issue (6): 1335-1343 Previous Articles   Next Articles

• Review •

Matrix-Assisted Laser Desorption/Ionization Mass Spectrometry(MALDI) Application in Carbohydrates Analysis

Wang Hongmin1|Zhang Ping1,2| Huang Linjuan1|Wang Zhongfu1**   

  1. (1. Key Laboratory of Ministry of Education for Western China Resource Biology and Biotechnology, Key Laboratory of Shanxi Province for Biotechnology, The College of Life Sciences, Northwest University, |Xi ’an 710069, China|2.Chemistry Departement of Xianyang Normal University, Xianyang 712000, China )
  • Received: Revised: Online: Published:
  • Contact: Wang Zhongfu E-mail:wangzhf@nwu.edu.cn
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Matrix-assisted laser desorption/ionization (MALDI) mass spectrometry (MS) is a rapid and high sensitivity method, with spectrum unscrambling easily and sample underivatized for structural elucidation of biological specimen. This technique has been widely used to analyze the structure of the glycan and glycoprotein. Combined with other methods such as high performance liquid chromatography ( HPLC ) or exoglycosidases digestion, MALDI-MS can conveniently provides more detailed information for analysis carbohydrate structure. With kinds of tandem mass spectrometry introduced in the recent years, high quality fragmentation spectra can be obtained now, offering the possibility of more complete analysis by mass spectrometry alone. The application of MALDI-MS to analyze carbohydrates and glycoprotein is reviewed in this article, the principle and characteristics of the instrument, the correlation technique and cleavage mode of MALDI combined with time-of-flight（TOF）analyser, MALDI matrix selection, sample preparation, fragmentation identification and glycosylation site definition are also discussed, and the prospect is showed at last. With the better maxtrix , higher resolution and wider molecular weight, MALDI-MS will play a role of significance in carbohydrates analysis.

Contents
1 Principles and characteristics of MALDI
2 MALDI combined with time-of-flight（TOF）analyser
2.1 Linear mode
2.2 Reflectron mode
3 Methods for MALDI -MS analysis of carbohydrates
3.1 Matrix selection
3.2 Sample preparation
3.3 Fragmentation identification
4 Applications of MALDI -MS to the carbohydrates analysis
4.1 Determination of molecular weight
4.2 Analysis of carbohydrates structure
4.3 Definition of glycosylation site
5 Prospect

Key words: matrix-assisted laser desorption/ionization mass spectrometry, carbohydrates, structures

CLC Number: 

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