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Progress in Chemistry 2009, Vol. 21 Issue (6): 1325-1334 Previous Articles   Next Articles

• Review •

Light Emitting Diode Induced Fluorescence Detector

Xu Jing; |Xiong Yan; |Chen Shiheng; |Guan Yafeng**   

  1. (Department of Instrumentation &|Analysis Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116023, China)
  • Received: Revised: Online: Published:
  • Contact: Guan Yafeng E-mail:guan_yafeng@yahoo.com.cn; guanyafeng@dicp.ac.cn
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Light emitting diode induced fluorescence detector (LED-IF) has been developed and widely used as detectors in capillary flow systems, including flow injection analysis, capillary liquid chromatography, capillary electrophoresis and microchip analysis. This review summarizes the progress of LED-IF in recent years. Four kinds of optical arrangements are presented. The main parts of LED-IF, including light source, filter, lens, optical fiber, photo electronic detector and detection cell, are discussed in detail. The applications of LED-IF in bioanalysis，pharmaceutical analysis and environmental analysis are presented. Furthermore, the trend of development LED- IF is also discussed.

Contents
1 Introduction
2 Optical setup
2.1 Confocal setup
2.2 Orthogonal setup
2.3 Intersectant setup
2.4 Parallel setup
3 Optical fittings
3.1 LED
3.2 Lens
3.3 Filters
3.4 Optical fibers
3.5 Optical detectors
3.6 Detection cell
4 Multifunctional detectors
5 Applications
6 Conclusions

Key words: instrument miniaturization, light emitting diodes, fluorescence, detectors, capillary flow systems

CLC Number: 

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