中文
Announcement
More
Progress in Chemistry 2009, Vol. 21 Issue (11): 2372-2378 Previous Articles   Next Articles

• Special issues •

Water-Soluble Conjugated Polymers for Protein Detections

He Fang ;   Wang Shu**   

  1. (Key Laboratory of Organic Solids, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China)
  • Received: Online: Published:
  • Contact: Wang Shu E-mail:wangshu@iccas.ac.cn
PDF ( 1579 ) Cited
Export

EndNote

Ris

BibTeX

Conjugated polymers (CPs) are characterized by a delocalized electronic structure. The excitation energy along the whole backbone of CPs transferring to the reporter results in the amplified fluorescence signal, which makes them be used as the optical platforms in highly sensitive chemical and biological sensors. This new technology is receiving more and more research and application interest due to the rapidly increasing demands of clinical diagnosis, environmental analysis and home-land defense. In this review, the mechanism of signal amplification of CPs and their applications in sensitive protein detection are introduced. Furthermore, future research directions for protein detections based on CPs are also presented.

Contents
1 Introduction
2 Mechanism of signal amplification of CPs
2.1. Conformation transition mechanism
2.2. Fluorescent signal amplification
3 CPs for protein detections
3.1 Protein detection based on conformation transition of CPs
3.2 Protein detection based on fluorescent signal amplification of CPs
4 Conclusion and prospects

CLC Number: 

[ 1 ]  Roncali J , Garreau R , Delabouglise D , Garnier F , Lemaire M.Chem. Commun. , 1989 , 679 —681
[ 2 ]  Shi L M, Garnier F , Roncali J . Synth. Met . , 1991 , 41P43 : 547 —550
[ 3 ]  McQuade D T, Pullen A E , Swager TM. Chem. Rev. , 2000 , 100 :2537 —2574
[ 4 ]  Leclerc M. Adv. Mater. , 1999 , 11 : 1491 —1498
[ 5 ]  Garnier F. Angew. Chem. Int . Ed. , 1989 , 28 : 513 —517
[ 6 ]  Leclerc M, Ho H A. Synlett , 2004 , 2 : 380 —387
[ 7 ]  Patel G N , Chance R R , Witt J D. J . Chem. Phys. , 1979 , 70 :4387 —4392
[ 8 ]  Elsenbaumer R L , Jen K Y, Oboodi R. Synth. Met . , 1986 , 15 :169 —174
[ 9 ]  Sato M A , Tanaka S , Kaeriyama K. Chem. Commun. , 1986 ,1346 —1347
[10 ]  Sugimoto R , Takeda S , Gu H B , Yoshino K. Chem. Express ,1986 , 1 : 635 —638
[11 ]  Hotta S , Rughooputh S D D V , Heeger A J , Wuld F.Macromolecules , 1987 , 20 : 212 —215
[12 ]  Leclerc M, Diaz F M, Wegner G. Makromol . Chem. , 1989 , 190 :3015 —3019
[13 ]  Leclerc M. J . Polym. Sci . Part A: Polym. Chem. , 2001 , 39 :2867 —2873
[14 ]  Bernier S , Garreau S , Bera-Aberem M, Gravel G, Leclerc M. J .Am. Chem. Soc. , 2002 , 124 : 12463 —12468
[15 ]  Tan C , Pinto M R , Kose M E , Ghiviriga I , Schanze K S. Adv.Mater. , 2004 , 16 : 1208 —1212
[16 ]  Stork M, Gaylord B S , Heeger A J , Bazan G C. Adv. Mater. ,2002 , 14 : 361 —366
[17 ]  Tan C , Pinto M R , Schanze S. Chem. Commun. , 2002 , 2 : 446 —447
[18 ]  Pinto M R , Kistal B M, Schanze S. Langmuir , 2003 , 19 : 6523 —6533
[19 ]  Prince R B , Saven J G, Wolynes P G, Moore J S. J . Am. Chem.Soc. , 1999 , 121 : 3114 —3121
[20 ]  Lahiri S , Thompson L J , Moore J S. J . Am. Chem. Soc. , 2000 ,122 : 11315 —11319
[21 ]  Zhou Q , Swager T M. J . Am. Chem. Soc. , 1995 , 117 : 7017 —7018
[22 ]  Zhou Q , Swager T M. J . Am. Chem. Soc. , 1995 , 117 :12593 —12602
[23 ]  Mullen K, Wegner G. Electronic Materials : The Oligomer Approach.Weinheim: Wiley-VCH , 1998
[24 ]  Thienpont H , Rikken GL J A , Meijer E E , Hoeve W, Wynberg H.Phys. Rev. Lett . , 1990 , 65 : 2141 —2144
[25 ]  Swager T M. Acc. Chem. Res. , 1998 , 31 : 201 —207
[26 ]  Chen L , Xu S , McBranch D , Whitten D. J . Am. Chem. Soc. ,2000 , 122 : 9302 —9303
[27 ]  Tan C , Pinto M R , Schanza K S. Chem. Commun. , 2002 , 446 —447
[28 ]  Lavigne J J , Broughton D L , Wilson J N , Erdogan B , Bunz U H F.Macromolecules , 2003 , 36 : 7409 —7412
[29 ]  Fan C , Wang S , Hong J W, et al . Proc. Natl . Acad. Sci . USA ,2003 , 100 : 6297 —6301
[30 ]  Ho H A , Leclerc M. J . Am. Chem. Soc. , 2004 , 126 : 1384 —1387
[31 ]  Tang Y, Feng F , He F , Wang S , Li Y, Zhu D. J . Am. Chem.Soc. , 2006 , 128 : 14972 —14976
[32 ]  Nilsson K P R , Rydberg J , Baltzer L , Ingan?s O. Proc. Natl .Acad. Sci . USA , 2003 , 100 : 10170 —10174
[33 ]  Nilsson K P R , Ingan?s O. Macromolecules , 2004 , 37 : 9109 —9114
[34 ]  Nilsson K P R , Herland A , Hammarstrom P , Ingan?s O.Biochemistry , 2005 , 44 : 3718 —3724
[35 ]  Chen L , McBrach D W, Wang HL , et al . Proc. Natl . Acad. Sci .USA , 1999 , 96 : 12287 —12292
[36 ]  Jones R M, Bergstedt T S , McBranch D W, Whitten D G. J . Am.Chem. Soc. , 2001 , 123 : 6726 —6727
[37 ]  Huang F , Wang X H , Wang D L , Yang W, Cao Y. Polymer , 2005 ,46 : 12010 —12015
[38 ]  An L , Tang Y, Wang S , Li Y, Zhu D. Macromol . Rapid Commun. , 2006 , 27 : 993 —997
[39 ]  Fan C , Plaxco K W, Heeger AJ . J . Am. Chem. Soc. , 2002 , 124 :5642 —5643
[40 ]  Moore G R , Pettigrew G W. Cytochromes C. Evolutionary , Structral and Physicochemical Aspects. Berlin : Springer-Verlag , 1990
[41 ]  Herzberg R P , Pope A J . Curr. Opin. Chem. Biol . , 2000 , 4 :445 —451
[42 ]  Eisenthal R , Danson M J . Enzyme Assays : A Practical Approach.Oxford : IRL Press at Oxford University , 1992
[43 ]  Pinto M R , Schanze K S. Proc. Natl . Acad. Soc. USA , 2004 ,101 : 7505 —7510
[44 ]  Talanian R V , Brady K D , Cryns V L. J . Med. Chem. , 2000 , 43 :3351 —3371
[45 ]  Earnshaw W C , Martins L M, Kaufman S H. Annu. Rev.Biochem. , 1999 , 68 : 383 —424
[46 ]  Kumaraswamy S , Bergstedt T, Shi X, et al . Proc. Natl . Acad.Soc. USA , 2004 , 101 : 7511 —7515
[47 ]  Feng F , Tang Y, Wang S , Li Y, Zhu D. Angew. Chem. Int . Ed. ,2007 , 46 : 7882 —7886
[48 ]  Nick J A , Yong S K, Brown K K, Avidi N J , Arndt P G, Worthen G S. J . Immunol . , 2000 , 164 : 2151 —2159
[49 ]  Vlahos C J , McDowell S A , Clerk A. Nat . Rev. Drug Discov. ,2003 , 2 : 99 —113
[50 ]  Antonsson A , Marshall C J , Montessuit S , Arkinstall S. Anal .Biochem. , 1996 , 267 : 294 —299
[51 ]  Angeles T S , Steffler C , Bartlett B A , Hudkins RL , Stephens RM,Kaplan D R , Dionne C A. Anal . Biochem. , 1996 , 236 : 49 —55
[52 ]  Xia W, Rininsland F , Wittenburg S K, et al . Assay Drug Dev.Technol . , 2004 , 2 : 183 —192
[53 ]  Andersson L , Porath J . Anal . Biochem. , 1986 , 154 : 250 —254
[54 ]  Chan D W, Perlstein M T. Immunoassay : A Practical Guide.Orlando : Academic Press , 1987
[55 ]  Wang D , Gong X, Heeger P S , Rininsland F , Bazan GC , Heeger A J . Proc. Natl . Acad. Sci . USA , 2002 , 99 : 49 —53
[56 ]  Kim I B , Dunkhorst A , Bunz U H F. Langmuir , 2005 , 21 : 7985 —7990
[57 ]  Kim I B , Dunkhorst A , Gilbert J , Bunz U H F. Macromolecules ,2005 , 38 : 4560 —4562
[58 ]  Song X D , Wang H L , Shi J , Park J W, Swanson B I. Chem.Mater. , 2002 , 14 : 2342 —2347
[59 ]  Thomas S W, Joly G D , Swager T M. Chem. Rev. , 2007 , 107 :1339 —1386
[60 ]  Feng F , He F , An L , Wang S , Li Y, Zhu D. Adv. Mater. , 2008 ,20 : 2959 —2964
[61 ]  Phillips R L , Miranda O R , You C C , Rotello V M, Bunz U H F.Angew. Chem. Int . Ed. , 2008 , 47 : 2590 —2594

[1] Zihan Lin, Huang Chen, Jiawei Dong, Daohui Zhao, Libo Li. Nanopore-Based Biomolecular Detection [J]. Progress in Chemistry, 2020, 32(5): 562-580.
[2] Yangyang Zhou, Jian Zhong, Xiaojun Bian, Gang Liu, Liang Li, Juan Yan. Application of Signal Amplification Technology in the Area of Food Safety Detection [J]. Progress in Chemistry, 2018, 30(2/3): 206-224.
[3] Sun Pengfei, Hou Huanzhi, Fan Quli, Huang Wei. Synthesis and Application of Water-Soluble Conjugated Glycopolymer [J]. Progress in Chemistry, 2016, 28(10): 1489-1500.
[4] Cai Xiaohui, Shi Lin, Liu Xingfen*, Huang Yanqin, Fan Quli, Huang Wei*. Functionalized Conjugated Polymers and Their Application in the Biological and/or Chemical Analysis [J]. Progress in Chemistry, 2013, 25(06): 975-989.
[5] Dong Haifeng, Zhang Xueji. DNA Biosensors Based on Functional Nanoprobes [J]. Progress in Chemistry, 2012, 24(11): 2247-2254.
[6] Yang Zhenglong, Bu Yilong, Chen Qiuyun. High Efficiency Low Band Gap Conjugated Polymer Materials for Solar Cells [J]. Progress in Chemistry, 2011, 23(12): 2607-2616.
[7] Zhu Chunlei, Yang Qiong, Liu Libing, Wang Shu. Conjugated Polymers for Sensitive Chemical Sensors [J]. Progress in Chemistry, 2011, 23(10): 1993-2002.
[8] Hu Juan, Zhang Chunyang. Surface-Enhanced Raman Scattering Technology and Its Application to Gene Analysis [J]. Progress in Chemistry, 2010, 22(08): 1641-1647.
[9] Li Di Zhang Long Fan Quli Huang Wei. Self-Assembly of Conjugated Rod-Coil Block Copolymers [J]. Progress in Chemistry, 2009, 21(12): 2660-2673.
[10] . Application of Fluorescent Conjugated Polymers in Detecting Biomacromolecules [J]. Progress in Chemistry, 2009, 21(04): 739-746.
[11] Feng Jichang, Li Yang, Yang Mujie*. Conjugated Polymers Based Fluorescent Chemosensors for ions [J]. Progress in Chemistry, 2008, 20(12): 2012-2020.
[12] Huang Yanqin|Fan Quli|Huang Wei**. Water-Soluble Conjugated Polyelectrolytes [J]. Progress in Chemistry, 2008, 20(04): 574-585.
[13] Li Yongfang. Conducting Polymers [J]. Progress in Chemistry, 2002, 14(03): 207-.