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Progress in Chemistry 2009, Vol. 21 Issue (09): 1895-1904 Previous Articles   Next Articles

• Review •

The Application of Microfluidic Chip Electrophoresis in Biomolecular Interaction Research

JiangPing; |Qu Feng; |Tan Xin; |Li Qin; |Geng Lina** |Deng Yulin**   

  1. (School of Life Science and Technology| Beijing Institute of Technology| Beijing 100081, China)
  • Received: Revised: Online: Published:
  • Contact: Geng Lina;Deng Yulin E-mail:lngeng@hotmail.com;deng@bit.edu.cn
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It is of great significance to deeply understand the life progress and promote medicine developments using appropriate techniques to characterize interaction between biomolecules. Microfluidic chip electrophoresis technique which combines microfluidic chip with capillary electrophoresis possesses many advantages including rapid, high performance, high throughput, low sample consumption and easy of integration, etc. It has already demonstrated great potential just in the early stage of application. In this paper, the separation mode, analysis methods and detection techniques in chip-CE biological binding constant and binding mechanism are reviewed respectively. The chip-CE is also simply compared with micro-array chip biomolecular interaction analysis. Finally, the development prospects and present situations in chip-CE are analyzed.

Contents
1 Introduction
2 Different microfluidic chip electrophoresis separation mode for biointeraction
2.1 Microfluidic chip zone migration electrophoresis
2.2 Microfluidic chip sieving electrophoresis
2.3 Microfluidic chip isoelectric focusing
2.4 Microchip electrochromatography
2.5 Microchip micellar electrokinetic chromatography
2.6 Microchip affinity electrophoresis
2.7 Microchip multichannel electrophoresis
3 Estimation of binding constants
4 Detection techniques of microfluidic chip electrophoresis
4.1 Laser-induced fluorescence detection
4.2 Electrochemical detection
4.3 Electrochemical luminescence detection
4.4 Mass spectrometry
4.5 UV absorbance detection
4.6 Surface plasmon resonance
5 Other techniques based on chip involved in biomolecular research
6 Conclusion and perspective

Key words: microfluidic chip electrophoresis, biomolecular interaction, separation mode, detection techniques

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