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Progress in Chemistry 2008, Vol. 20 Issue (0708): 1158-1164 Previous Articles   Next Articles

• Review •

Techniques for Staining Glycoprotein on Gel Electrophoresis or Electroblotting

Zhang Ying; Huang Linjuan; Wang Zhongfu**

  

  1. （Key Laboratory of Resource Biology and Biotechnology in Western China, Ministry of Education, Shaanxi Provincial Key Laboratory of Biotechnology, Life Science College, Northwest University, Xi'an 710069, China）
  • Received: Revised: Online: Published:
  • Contact: Wang Zhongfu
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Glycosylation is one of the most important protein post-translational modification methods, which is essential to life and function of proteins. Two-dimensional gel electrophoresis (2-DE) is one of the most commonly used methods developed for the separation of proteins, and identification of glycoproteins in gel is a key step for the separation and purification of glycoproteins. This review gives an overview of derivatization reagents for detecting of glycoprotein on gel electrophoresis or electroblotting, and the reaction mechanisms of most derivative reagents are also introduced.
Key words: glycoprotein, gel electrophoresis, electroblotting, staining

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