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Progress in Chemistry 2008, Vol. 20 Issue (05): 754-761 Previous Articles   Next Articles

• Review •

Protein/peptides Separation by Two-dimensional Microfluidic chip Electrophoresis

Xu Yi1,2,3** Shen Jiwei1,2 Lu Jiali1,2 Wen Zhiyu1,3

  

  1. (1. Micro System Research Center, Chongqing University, Chongqing 400044, China; 2. Chemistry and Chemical Engineering College, Chongqing University, Chongqing 400044, China; 3. Key Lab for Optoelectronic Technology & Systems of Ministry of Education, Chongqing University, Chongqing 400044, China)
  • Received: Revised: Online: Published:
  • Contact: Xu Yi
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A powerful tool is presented in the proteome studies by muti-dimensional separation system constructed on the micofluidic chip platform. Approaches to estimate the orthogonality between selected separation modes are presented in this paper. Microfluidic two-dimensional chip electrophoresis system of various coupling modes, such as micellar electrokinetic chromatography (MEKC) coupled with capillary zone electrophoresis (CZE), open-channel electro-chromatography (OCEC)-CZE, isoelectric focusing (IEF)-CZE, IEF-SDS-capillary gel electrophoresis (CGE), SDS-CGE-MEKC, etc., designed for protein/peptides separation are summarized. Particularly, types of switching interface for two-dimensional electrophoresis microchip are classified and detecting techniques for two-dimensional microchip electrophoresis are discussed. Application prospects and developing trends of microfluidic two-dimensional electrophoresis chip in the proteome studies are also discussed.

Key words: microfluidic chip, two-dimensional electrophoresis, protein/peptides separation

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