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Progress in Chemistry 2008, Vol. 20 Issue (01): 163-170 Previous Articles   Next Articles

• Review •

Analyzing the Effect of Carrier on the Microenvironment of Encapsulated Enzyme

Xu Songwei 1*; Zhang Jie1; Yang Zhanping1; Cao Jianhua 1; Ma Xiaolong1; Jiang Zhongyi2   

  1. (1. Technical Center, Nantong Cellulose Fibers Co., Ltd., Nantong, Jiangsu 226008, China;
    2. School of Chemical Engineering &Technology, Tianjin University, Tianjin 300072, China)
  • Received: Revised: Online: Published:
  • Contact: Xu Songwei
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It is very important to select and design the novel carriers for enzyme encapsulation, especially to create the appropriate microenvironment for encapsulated enzymes in the carriers. The key factors affecting the enzyme microenvironment in the carrier for enzyme encapsulation were analyzed and proposed in this review, including the hydrophilicity, the structure and morphology, and the reactivity of the carriers. Water content in the carrier is determined by its hydrophilicity. The enzyme stability and conformational transitions are impacted by the cage effect formed by the carrier structure and morphology, and by the reactivity of the carriers, such as the activity of covalent linking, hydrogen bonding and electrostatic interaction. Meanwhile, the diffusion properties of substrate/product and the enzyme accessibility are affected by the pore structure and distribution, and by the reactivity of carriers. Then encapsulation processes for normally used carriers are presented, including sol-gel silica, polymer hydrogel and polymer-silica hybrid composite. According to the factors mentioned above, the enzyme loading efficiency, catalytic activities, and stability used the three types of carriers for enzyme encapsulation were analyzed. Also promotions to modify the microenvironment of encapsulated enzymes in the three types of carrier are discussed.
Key words: enzyme encapsulation, microenvironment, polymer, sol-gel, silica, hybrid composites

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