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Progress in Chemistry 2007, Vol. 19 Issue (05): 633-642   Next Articles

• Invited Article •

Fluorescent Labeling Reagents and Molecular Probes used in Separation and Detection of Bioactive Substances

Hong Wang; Huashan Zhang**   

  1. College of Chemistry & Molecular Science,WuhanUniversity,Wuhan,430072,China
  • Received: Revised: Online: Published:
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Integrated with the authors' work，the recent development and application of the fluorescent labeling reagents and molecular probes for the determination of amino acids, peptides, proteins, biological amines, thiol compounds and NO etc, are reviewed. Besides OPA, NDA, DNS, FMOC, FITC, NBD-F, AQC, Cy5, some recent fluorescent label reagents and molecular probes are also discussed with respect to their application in the separation and fluorescent detection of HPLC or CE. They are N-hydrxylsuccinimidyl ester fluorescent labeling reagent, 6-oxo- N-hydroxysuccinimidyl acetic acid ester)-9-(2'-methyloxocabonyl) fluorescein, 8-phenyl-(4'-oxy-acetic acid N-hydroxysuccinimidyl ester)-4, 4-difluoro-1, 3, 5, 7-tetramethyl-4-bora-3a, 4a-diaza-S-indacene (TMPAB-OSu) etc , fluorescent probe 3-iodoacemidobenzanthranone and near-infrared fluorescent probe MCY5, DSTCY、DCDSTCY and DCTCY.
Key words: labeling reagents, fluorescent molecular probes, fluorescent detection, HPLC, CE, image

CLC Number: 

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