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Progress in Chemistry 2006, Vol. 18 Issue (0708): 987-994 Previous Articles   Next Articles

• Review •

Mechanisms of DNA Separation by Capillary Electrophoresis in Non-Gel Sieving Matrices

Dan Zhou;Yanmei Wang**   

  1. Department of Polymer Science and Engineering, University of Science and Technology of China, Hefei 230026, China

     

  • Received: Revised: Online: Published:
  • Contact: Yanmei Wang
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Fast, highly efficient, and sensitive separation technique is crucial for DNA analysis. The capillary electrophoresis using non-gel sieving matrices is one of the most important techniques for DNA separation. The uncrosslinked polymer solutions are generally used as sieving matrices. Based on the introduction of the theory of the polymer solutions, the mechanisms of DNA separation by the capillary electrophoresis in non-gel sieving matrices (entangled and dilute solutions) are reviewed in this paper, which includes Ogston sieving model, various modified reptation models, transient entanglement coupling mechanisms and its improved mechanisms.
Key words: capillary electrophoresis, DNA separation, non-gel sieving matrices, polymer solutions, mechanisms

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