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Progress in Chemistry 2006, Vol. 18 Issue (04): 474-481 Previous Articles   Next Articles

• Review •

Quantitative Analytical Techniques of Proteins Based on Two-Dimensional Gel Electrophoresis

Xiaohui Long1;Zhihong Mo2;Yaozhou Zhang3**   

  1. 1.College of Life Science, Zhejiang University, Hangzhou 310029, China; 2.College of Chemistry and Engineering, Chongqing University, Chongqing 400044, China; 3.College of Life Science, Zhejiang University of Science and Technology, Hangzhou 310018, China
  • Received: Revised: Online: Published:
  • Contact: Xiaohui Long
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Quantitative analytical techniques of proteins based on two-dimensional gel electrophoresis，depending on the comparing of altered levels of proteins between different samples to obtain quantitative changes, are informative for comprehesive understanding functions and biological mechanism of proteins in cells.Conventional methods in which protein samples are separated on individual gels are introduced, and shortcomings of these methods that limit their application in general 2DE analysis are discussed. More approachs including differential in-gel electrophoresis (DIGE), “differential exposure" approach, and stable isotope approach are stated. The latter methods permitting separation on a single gel containing mixed samples, have superior power in quantitative analysis compared to conventional methods. Various quantitative protocols, advantages and limitations of these protocols as well as further development of quantitative techniques based on 2DE are discussed.
Key words: two-dimensional gel electrophoresis, quantitative techniques, DIGE, isotope-coded method

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