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Progress in Chemistry 2006, Vol. 18 Issue (0203): 349-354 Previous Articles   Next Articles

• Review •

Directed Evolution of Enzymes in vitro and the Application in the Synthesis of Chiral Organic Compounds

Rong Kong;Lixi Niu;Jingming Yuan **   

  1. Key Laboratory of Chemical Biology and Molecular Engineering of National Ministry of Education, Institute of Biotechnology, Shanxi University, Taiyuan 030006 
  • Received: Revised: Online: Published:
  • Contact: Jingming Yuan
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The directed evolution technique, an effective method to improve the catalytic activity of enzymes and the basic principle such as error-prone PCR and DNA shuffling as well as some derivative techniques are reviewed. In addition, several successful examples in the synthesis of chiral organic compounds using directed evolution technique are also described.
Key words: enzyme molecules, directed evolution in vitro, chiral compounds, synthesis

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