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Progress in Chemistry DOI: 10.7536/PC240304   

Advances in intracellular single strand DNA research and high-throughput analysis techniques

Ruiqi Li1,2, Weiyi Lai1, Hailin Wang1,2,*   

  1. 1. Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China;
    2. Hangzhou Institute for Advance Study, University of Chinese Academy of Sciences, Hangzhou 310024, China
  • Received: Revised:
  • Contact: *e-mail: hlwang@rcees.ac.cn
  • Supported by:
    National Natural Science Foundation of China (No. 22234008,21927807,22021003,22274166).
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During many life processes such as replication, transcription, and double-strand breaks repair and so on, double-stranded DNA will temporarily unwind and form single strand DNA (ssDNA). ssDNA may affect genomic stability and may also participate in the formation of non-B DNA structure, which in turn regulates and influences many key cellular processes. This review briefly describes the causes of the formation of single-stranded DNA, the structures containing single-stranded DNA and their possible functions in cells, and summarizes some high-throughput analysis techniques of single-stranded DNA, which provides the method inspiration for the subsequent ssDNA research and promotes the further development of ssDNA analysis techniques and methods．
Key words: single strand DNA, R-loop, G-quadruplex, high-throughput sequencing
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