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Progress in Chemistry 2023, Vol. 35 Issue (2): 219-232 DOI: 10.7536/PC220723 Previous Articles   Next Articles

• Review •

Progress in Electrochemical Sensing of Ascorbic Acid

Yanyu Zhong, Zhengyun Wang(), Hongfang Liu()   

  1. School of Chemistry and Chemical Engineering, Huazhong University of Science and Technology, Key Laboratory of Material Chemistry for Energy Conversion and Storage, Hubei Key Laboratory of Materials Chemistry & Service Failure,Wuhan 430074, China
  • Received: Revised: Online: Published:
  • Contact: *e-mail: wzyhustsh@163.com(Zhengyun Wang); liuhf@hust.edu.cn(Hongfang Liu)
  • Supported by:
    National Natural Science Foundation of China(52171069); National Natural Science Foundation of China(22005109); China Postdoctoral Science Foundation(2021M701292)
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Ascorbic acid (AA), as a necessary biological small molecular substance to maintain the normal function of human body, directly and indirectly participates in many key biological reaction processes of human body. Electrochemical detection of AA has been a hot spot of sensing research in recent years due to the advantages such as fast response, high sensitivity and simple operation. In this review, the working principles of different sensors of AA are systematically and comprehensively introduced, and the recent research progress of electrochemical sensing of AA is reviewed. By comparing different materials based AA sensors performances, the advantages and defects of corresponding materials are analyzed and summarized by combining with the materials properties. Lastly, the outlook of development direction and trend of AA electrochemical sensing is given.

Contents

1 Introduction

2 Principles of electrochemical detection of ascorbic acid

3 Enzymatic sensors

4 Non-enzymatic sensors

4.1 Metal-based sensor

4.2 Conductive polymer-based sensor

4.3 Carbon material-based sensor

5 Conclusion and outlook

Key words: ascorbic acid, electrochemical sensor, enzymatic, nonenzymatic
Fig.1 Chemical structure and redox reaction process of AA
Fig.2 Electrocatalytic reaction process (enzyme sensor: M: complex of AA and ascorbic acid oxidase, N: ascorbic acid oxidase; nonenzymatic senor: M: catalytic material, N: activated catalytic material)
Fig.3 Ascorbic acid determination in stimulated sweat[19]. Copyright 2020, American Chemical Society
Fig.4 (a) The principle of immobilized enzyme[22]; (b) the electron transfer process of enzyme active site[21]. Copyright 2007, WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
Fig.5 Schematic of the wearable vitamin C sensor for nutritional assessment[32]. Copyright 2020, Wiley-VCH GmbH
Fig.6 (A) SEM image of Au NPs/RGO/SPCEs; (B) CVs recorded at the AuNPs/RGO/SPCEs and bare SPCEs; (C) CVs recorded from -0.10 to +0.70 V, at different scan rate; (D) plot of iap of B vs ν1/2 and plot of Eap of AA vs logν[39]. Copyright 2020, Elsevier Ltd.
Fig.7 (a) Structure and (b) electrochemical activity of POMOFs[47]. Copyright 2020, Elsevier Ltd.
Fig.8 (a) Block diagram of part of the circuitry in the portable enzyme-free AA detection system. (b) Conceptual diagram of sensor devices for non-invasive human health monitoring[57]. Copyright 2020, Elsevier Ltd.
Fig.9 (a) The doping mechanism of PANI proton acid[61]; (b) the reaction mechanism between PANI and AA[63]; (c) preparation process of PANI/MnO2-Sb2O3 /FTO[69]. Copyright 2020, Elsevier Ltd.
Fig.10 (a) Preparation of the different pTS-/PPy MIPs by electropolymerization of Py in the presence of the template molecules and (b) incorporation of the fabricated MIP-based sensors into the ET sensor array and its application to the analysis of APIs in pharmaceutical samples[74]. Copyright 2021, Elsevier B.V.
Fig.11 Working principle and electrochemical performance of ratio electrochemical sensor[79]. Copyright 2020, American Chemical Society
Fig.12 (a) Process diagram of constructing AA electrochemical sensor based on 3D-KSC/CCSBP material and (b) morphology of 3D-KSC/CCSBP[92]. Copyright 2021, Elsevier B.V.
Table 1 Advantages and deficiency of AA sensors based on different materials
Sensing materials Advantage Deficiency
Enzymatic electrode Highly sensitive and selective,wide liner range,good reproducibility High cost, poor long-term stability, oxygen dependent, tedious purification and meticulous immobilization process
Metal-based electrode High sensitive,fast response, wide liner range, low detection limit, stable, selective Complex preparation process, indeterminate structure-activity relationship
Conductive polymer-based electrode Sensitive, wide liner range, stable, comparatively low cost, selective, tunable High overpotential and poor detection limit
Carbon material-based electrode Good biocompatibility, fast response, scalable production, wide liner range, sensitive Poor selectivity, high overpotential
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