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Progress in Chemistry 2019, Vol. 31 Issue (8): 1129-1135 DOI: 10.7536/PC190201 Previous Articles   Next Articles

Design and Application of Electrochemical Sensor in Cell Detection

Ye Xia1,2,4, Xi Su1,2,4, Li Chen1,3,4,5, Shunbo Li1,3,4,5,**(), Yi Xu1,2,3,4,5,**()   

  1. 1. Key Disciplines Laboratory of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400030, China
    2. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China
    3. Key Laboratory for Optoelectronic Technology & System of Ministry of Education, Chongqing University, Chongqing 400030, China
    4. International R & D Center of Micro-Nano Systems and New Materials Technology, Chongqing University, Chongqing 400030, China
    5. School of Optoelectronics Engineering, Chongqing University, Chongqing 400030, China
  • Received: Online: Published:
  • Contact: Shunbo Li, Yi Xu
  • About author:
    ** E-mail: (Yi Xu)
    (Shunbo Li)
  • Supported by:
    National Natural Science Foundation of China(61971074); National Natural Science Foundation of China(61904021); Chongqing Arpngicial Intelligence Technology Innovation Major Special Project(CSTC2017RGZN-ZDYFX0019); Major Project of Technological Innovation and Application Demonstration of Chongqing(CSTC2018JSZX-CYZTZX0216); Special Funds for Basic Scienpngic Research in Central Universities(2019CDYGYB003)
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The application and development of electrochemical sensors and sensing methods in the field of cell detection are introduced briefly, aiming at the core problems in cell detection, such as detection of tumor cell activity, neurotransmitters in nerve cells and oxidative damage in macrophages. The design, fabrication, detection and application of electrochemical sensors with different microelectrode structures are discussed in details. It is shown that electrochemical sensors have developed from single detection electrode to integrated multi-functional and array electrode, and from single electrode sensing detection mode to chip integrated microelectrode sensing system. However, its biocompatibility, detection limit and detection efficiency need to be further improved and expanded. The development of microelectrode based on MEMS(Micro-electro-mechanical system)technology, the research of various chemical and biological modified sensitive film on the electrode surface, the material expansion from silicon-based to polymer flexible base electrode, and the development of small volume, implantable and wearable electrochemical and biochemical sensors are the current development directions. It has also illustrated great application prospects in clinical examination, precision medicine, sports health monitoring, elderly health services and many other fields.

Key words: cell detection, microelectrodes, micro-electro-mechanical system technique, reactive oxygen, flexible electrode
Fig. 1 Development of Clark sensors.(A)Calibration diagram of traditional Clark electrochemical sensor;(B)All solid state dissolved oxygen sensor[18,19]
Fig. 2 Sensor diagram of hydrogen peroxide detection array chip[27]
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