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Progress in Chemistry 2016, Vol. 28 Issue (5): 697-710 DOI: 10.7536/PC151118 Previous Articles   Next Articles

• Review and comments •

Microcantilever Biosensors

Dai Yingping1, Ji Zhengping1, Wang Chengyin1*, Hu Xiaoya1*, Wang Guoxiu2   

  1. 1. College of Chemistry and Engineering, Yangzhou University, Yangzhou 225002;
    2. School of Mathematical and Physical Sciences, University of Technology Sydney, NSW 2007, Australia
  • Received: Revised: Online: Published:
  • Supported by:
    The work was supported by the National Natural Science Foundation of China (No. 21375116), the National High Teehnology Research and Development Program of China (No.2009AA03Z331) and the Research Program on Analytical Methods and Techniques on the Shared Platform of Large-Scale Instruments and Equipment in Jiangsu Province(BZ201409).
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Microcantilever biosensors based on atomic force microscope have the advantages of label-free, rapid, real-time, and high sensitivity detection features. Therefore, microcantilever biosensors can be applied in the fields of biomedicine, environmental monitoring, food production and military defenses. Microcantilever biosensors have become the focus of scientific research. In this paper, we review the working principle, excitation method and detection mechanism of microcantilever biosensors. Preview reviews about the microcantilever biosensors mainly focuse on the progress of applications, but lack of comprehensive and systematic introduction on detection methods. Herein, we not only systematically analyze eight detection methods, but also introduce several typical sizes, appearance and "heat mode" of microcantilever biosensors. So far, these have not been reported in literatures. In order to realize specific detection and improve sensitivity of microcantilever sensors, we summarize the surface modification methods of microcantilever sensors and review the latest applications of microcantilever biosensors. Furthermore, we introduce a new kind of self-actuating and self-sensing microcantilever biosensors. The prospective of microcantilever biosensors is also discussed in this paper.

Contents
1 Introduction
2 Microcantilever biosensors
3 The brief introduction of microcantilever beam
4 Microcantilever working mode
4.1 Dynamic working mode
4.2 Static working mode
4.3 Heat mode
5 Excitation methods of the microcantilever biosensors
6 Detection methods of the microcantilever biosensors
6.1 Optical lever
6.2 Optical interferometry
6.3 Piezoresistive method
6.4 Piezoelectric method
6.5 Capacitance method
6.6 Electron tunneling method
6.7 Other methods
7 Surface biofunctionalization
8 Application of the microcantilever biosensors
8.1 DNA hybridization detection
8.2 Pathogens detection
8.3 Protein detection
8.4 Small-molecular and drug testing
8.5 Biological warfare agent detection
8.6 Self-actuating and self-sensing microcantilever biosensors
9 Conclusion and outlook

Key words: microcantilever, biosensor, atomic force microscope, detection, application

CLC Number: 

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Abstract

Microcantilever Biosensors