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Progress in Chemistry 2015, Vol. 27 Issue (12): 1799-1807 DOI: 10.7536/PC150636 Previous Articles   Next Articles

• Review and comments •

The Application of DNA Biosensor Based on Conjugated Polymers

Ma Yun, Zhou Yan, Du Wenqi, Miao Zhihui, Qi Zhengjian*   

  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
  • Received: Revised: Online: Published:
  • Supported by:
    The work was supported by the Jiangsu Province Transformation of Scientific and Technological Achievements Program (No. BA2014123) and the National Major Scientific Instruments and Equipment Development Projects(No. 2014YQ060773).
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Conjugated polymers, with π electron systems and highly delocalized conjugated structures, exhibit excellent luminescence properties. The polymer chains can work as molecular wire, which will lead to the amplification of optical signals and thus improve the detecting sensitivity. Aptamer has advantages in specificity, affinity with targets and signal transmission, hence, nucleic acid biosensors based on conjugated polymers have witnessed a rapid development in bio-detection. The applications of nucleic acid biosensors based on conjugated polymers in recent years are summarized. Finally, an outlook of the developing trend for these sensors is given.

Contents
1 Introduction
2 The sensing mechanism of fluorescent sensor
3 FRET
3.1 Detection of complementary DNA
3.2 Real-time monitoring of DNA hybridization
3.3 Real-time monitoring of DNA structure
3.4 Detection of protein and the activity of enzyme
3.5 Detection of specific gene
4 Aggregation and conformation change
4.1 Detection of complementary DNA
4.2 Real-time monitoring of DNA hybridization
4.3 Real-time monitoring of DNA structure
4.4 Detection of enzyme
4.5 Detection of specific DNA sequence
4.6 Detection of metal ion
5 Superquenching
6 Conclusion

Key words: conjugated polymer, aptamer, biological detection, FRET, conformation change

CLC Number: 

[1] Gaylord B S, Heeger A J, Bazan G C. Proc. Natl. Acad. Sci. U.S.A., 2002, 99(17): 10954.
[2] Liu Y, Ogawa K, Schanze K S. J. Photoch. Photobio. C, 2009, 10(4): 173.
[3] McQuade D T, Pullen A E, Swager T M. Chem. Rev., 2000, 100(7): 2537.
[4] Burrezo P M, Pelado B, Ortiz R P, De la Cruz P, Navarrete J T L, Langa F, Casado J. Chem.Eur. J., 2015, 21(4): 1713.
[5] Farcas A, Aubert P H, Mohanty J, Lazar A I, Cantin S, Nau W M. Eur. Polym. J., 2015, 62: 124.
[6] Farcas A, Resmerita A M, Aubert P H, Ghosh I, Cantin S, Nau W M. Macromol. Chem. Phys., 2015, 216(6): 662.
[7] Ghosh D, Chattopadhyay N. J. Lumin., 2015, 160: 223.
[8] Li P L, Di Stasio F, Eda G, Fenwick O, McDonnell S O, Anderson H L, Chhowalla M, Cacialli F. ChemPhysChem, 2015, 16(6): 1258.
[9] Bunka D H J, Stockley P G. Nat. Rev. Microbiol., 2006, 4(8): 588.
[10] Bigwood D W, Heller S R, Wolf W R, Schubert A, Holden J M. Anal. Chim. Acta, 1987, 200(1): 411.
[11] Gold L. Harvey lectures, 1995, 91: 47.
[12] Jayasena S D. Clin. Chem., 1999, 45(9): 1628.
[13] Klug S J, Famulok M. Mol. Biol. Rep., 1994, 20(2): 97.
[14] Liu X F, Fan Q L, Huang W. Biosens. Bioelectron., 2011, 26(5): 2154.
[15] Huang Y Q, Fan Q L, Zhang G W, Chen Y, Lu X M, Huang W. Polymer, 2006, 47(15): 5233.
[16] Li J, Huang Y Q, Qin W S, Liu X F, Huang W. Polymer Chemistry, 2011, 2(6): 1341.
[17] Lan M H, Liu W M, Wang Y, Ge J C, Wu J S, Zhang H Y, Chen J H, Zhang W J, Wang P F. ACS Appl. Mater. Interfaces, 2013, 5(6): 2283.
[18] 支俊格(Zhi J G), 徐秀玲(Xu X L), 申进波(Shen J B), 赵玮(Zhao W), 佟斌(Tong B), 董宇平(Dong Y P). 化学进展(Prog.Chem.), 2009, (04): 739.
[19] Liu R X, Tan Y, Zhang C L, Wu J T, Mei L, Jiang Y Y, Tan C Y. J. Mater. Chem. B, 2013, 1(10): 1402.
[20] 黄艳琴(Huang Y Q), 范曲立(Fan Q L), 黄维(Huang W). 化学进展(Prog.Chem.), 2008, (04): 574.
[21] 汪凌云(Wang L Y), 曹德榕(Cao D R). 化学进展(Prog.Chem.), 2010, (05): 905.
[22] Liu B, Wang S, Bazan G C, Mikhailovsky A. J. Am. Chem. Soc., 2003, 125(44): 13306.
[23] Hwang D H, Lee J D, Kang J M, Lee S, Lee C H, Jin S H. J. Mater. Chem., 2003, 13(7): 1540.
[24] Huang Y Q, Fan Q L, Lu X M, Fang C, Liu S J, Yu-Wen L H, Wang L H, Huang W. Journal of Polymer Science Part A-Polymer Chemistry, 2006, 44(19): 5778.
[25] Bumagin N A, Sukhomlinova L I, Luzikova E V, Tolstaya T P, Beletskaya I P. Tetrahedron. Lett., 1996, 37(6): 897.
[26] Liang Z, Cabarcos O M, Allara D L, Wang Q. Adv. Mater., 2004, 16(9/10): 823.
[27] Furukawa Y, Cha Y H, Noguchi T, Ohnishi T, Tasumi M. Journal of Molecular Structure, 2000, 521: 211.
[28] Sakaguchi T, Sato M, Hashimoto T. Polymer, 2013, 54(9): 2272.
[29] Herland A, Nilsson K P R, Olsson J D M, Hammarstrom P, Konradsson P, Inganas O. J. Am. Chem. Soc., 2005, 127(7): 2317.
[30] Nilsson K P R, Olsson J D M, Stabo-Eeg F, Lindgren M, Konradsson P, Inganas O. Macromolecules, 2005, 38(16): 6813.
[31] Ho H A, Boissinot M, Bergeron M G, Corbeil G, Dore K, Boudreau D, Leclerc M. Angew. Chem. Int. Ed., 2002, 41(9): 1548.
[32] Gaylord B S, Heeger A J, Bazan G C. J. Am. Chem. Soc., 2003, 125(4): 896.
[33] He F, Tang Y L, Yu M H, Wang S, Li Y L, Zhu D B. Adv. Funct. Mater., 2007, 17(6): 996.
[34] Yu M H, Tang Y L, He F, Wang S, Zheng D G, Li Y H, Zhu D B. Macromol. Rapid Commun., 2006, 27(20): 1739.
[35] Xing C F, Yu M H, Wang S, Shi Z Q, Li Y L, Zhu D B. Macromol. Rapid Commun., 2007, 28(3): 241.
[36] Yu M H, He F, Tang Y, Wang S, Li Y L, Zhu D B. Macromol. Rapid Commun., 2007, 28(12): 1333.
[37] An L L, Tang Y L, Wang S, Li Y L, Zhu D B. Macromol. Rapid Commun., 2006, 27(13): 993.
[38] Duan X R, Li Z P, He F, Wang S. J. Am. Chem. Soc., 2007, 129(14): 4154.
[39] Zhou R Y, Xu C, Dong J, Wang G J. Biosens. Bioelectron., 2015, 65: 103.
[40] Liu Z W, Wang H L, Cotlet M. Chem. Mater., 2014, 26(9): 2900.
[41] Wang B, Yang Q, Liu L B, Wang S. Colloid Surf. B-Biointerfaces, 2011, 85(1): 8.
[42] Pu K Y, Liu B. Adv. Funct. Mater., 2009, 19(9): 1371.
[43] Feng X L, Duan X R, Liu L B, An L L, Feng F D, Wang S. Langmuir, 2008, 24(21): 12138.
[44] Wang C, Tang Y L, Liu Y, Guo Y. Anal. Chem., 2014, 86(13): 6433.
[45] Wang C, Tang Y L, Guo Y. ACS Appl. Mater. Interfaces, 2014, 6(23): 21686.
[46] Lian S, Liu C H, Zhang X B, Wang H H, Li Z P. Biosens. Bioelectron., 2015, 66: 316.
[47] Wang J, Liu B. Chem. Commun., 2009, (17): 2284.
[48] Wang Y Y, Zhang Y, Liu B. Anal. Chem., 2010, 82(20): 8604.
[49] Wang Y Y, Liu B. Biosens. Bioelectron., 2009, 24(11): 3293.
[50] Wang Y Y, Liu B. Langmuir, 2009, 25(21): 12787.
[51] Huang Y Q, Yao X, Zhang R, Lang O Y, Jiang R C, Liu X F, Song C X, Zhang G W, Fan Q L, Wang L H, Huang W. ACS Appl. Mater. Interfaces, 2014, 6(21): 19144.
[52] An L L, Liu L B, Wang S. Biomacromolecules, 2009, 10(2): 454.
[53] Zhan R Y, Fang Z, Liu B. Anal. Chem., 2010, 82(4): 1326.
[54] Sommers C D, Keire D A. Anal. Chem., 2011, 83(18): 7102.
[55] Sommers C D, Mans D J, Mecker L C, Keire D A. Anal. Chem., 2011, 83(9): 3422.
[56] Sommers C D, Ye H P, Kolinski R E, Nasr M, Buhse L F, Al-Hakim A, Keire D A. Anal. Bioanal. Chem., 2011, 401(8): 2445.
[57] Lan M H, Wu J S, Liu W M, Zhang W J, Ge J C, Zhang H Y, Sun J Y, Zhao W W, Wang P F. J. Am. Chem. Soc., 2012, 134(15): 6685.
[58] Guo Y Q, Chen Y X, Wei Y L, Li H H, Dong C. Spectrochim. Acta, 2015, 136: 1635.
[59] Li C, Numata M, Takeuchi M, Shinkai S. Angew. Chem. Int. Ed., 2005, 44(39): 6371.
[60] Nilsson K P R, Herland A, Hammarstrom P, Inganas O. Biochemistrys, 2005, 44(10): 3718.
[61] Plante M P, Berube E, Bissonnette L, Bergeron M G, Leclerc M. ACS Appl. Mater. Interfaces, 2013, 5(11): 4544.
[62] Lan M H, Liu W M, Ge J C, Wu J S, Sun J Y, Zhang W J, Wang P F. Spectrochim. Acta, 2015, 136: 871.
[63] Guan H L, Zhou P, Zeng S, Zhou X L, Wang Y, He Z K. Talanta, 2009, 79(2): 153.
[64] Ma Y, Xia Y, Yan L Q, Wang F, Miao Z H, Cui M F, Yao H T, Qi Z J, Anal. Methods, 2015, 7: 5814.
[65] Liu M, Li B X, Cui X. Biosens. Bioelectron., 2013, 47: 26.
[66] Wang L H, Liu X F, Yang Q, Fan Q L, Song S P, Fan C H, Huang W. Biosens. Bioelectron., 2010, 25(7): 1838.
[67] Ren X S, He F, Xu Q H. Asian J. Chem., 2010, 5(5): 1094.
[68] Ho H A, Leclerc M. J. Am. Chem. Soc., 2004, 126(5): 1384.
[69] Jia Y M, Zuo X L, Lou X D, Miao M, Cheng Y, Min X H, Li X C, Xia F. Anal. Chem., 2015, 87(7):3890.
[70] Liu Z, Wang H L, Cotlet M. Chem. Commun., 2014, 50(77): 11311.
[71] Xing X J, Zhou Y, Liu X G, Tang H W, Pang D W. Analyst, 2013, 138(21): 6301.
[72] Liu X F, Tang Y L, Wang L H, Zhang J, Song S P, Fan C H, Wang S. Adv. Mater., 2007, 19(13): 1662.
[73] Qin C J, Wong W Y, Wang L X. Macromolecules, 2011, 44(3): 483.
[74] Fan C H, Wang S, Hong J W, Bazan G C, Plaxco K W, Heeger A J. Proc. Natl. Acad. Sci. U.S.A., 2003, 100(11): 6297.
[75] Jones R M, Lu L D, Helgeson R, Bergstedt T S, McBranch D W, Whitten D G. Proc. Natl. Acad. Sci. U.S.A., 2001, 98(26): 14769.
[76] Lu L D, Helgeson R, Jones R M, McBranch D, Whitten D. J. Am. Chem. Soc., 2002, 124(3): 483.
[77] Lu L D, Jones R M, McBranch D, Whitten D. Langmuir, 2002, 18(20): 7706.
[78] Kushon S A, Bradford K, Marin V, Suhrada C, Armitage B A, McBranch D, Whitten D. Langmuir, 2003, 19(16): 6456.
[79] Srinivas A R G, Peng H, Barker D, Travas-Sejdic J. Biosens. Bioelectron., 2012, 35(1): 498.
[80] Srinivas A R G, Barker D, Travas-Sejdic J. Int. J. Nanotechnol., 2014, 11(5/8): 645.
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