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Progress in Chemistry 2015, Vol. 27 Issue (8): 1102-1109 DOI: 10.7536/PC150232 Previous Articles   Next Articles

Special Issue: 酶化学

Rational Design of Artificial Hydrolases in Protein Scaffolds

Zhao Yuan1, Zeng Jin1, Lin Yingwu*1,2   

  1. 1. School of Chemistry and Chemical Engineering, University of South China, Hengyang 421001, China;
    2. Laboratory of Protein Structure and Function, University of South China, Hengyang 421001, China
  • Received: Revised: Online: Published:
  • Supported by:
    The work was supported by the National Natural Science Foundation of China (No. 31370812), the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry, and the Natural Science Founds for Distinguished Young Scholar of Hunan Province (No. 2015JJ1012).
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Proteins are an important part of life, of which enzymes perform vital roles in biological systems. Rational protein design is a key approach for investigating the structure and function relationship of enzymes. This review summarizes the progresses in rational design and functional study of artificial hydrolases in protein scaffolds, including reuse of natural proteins, reconstruction of natural proteins, molecular design based on 3-α-helix, 4-α-helix or zinc-finger proteins, and fine-tuning the hydrolysis activity of heme proteins such as cytochrome b5 and myoglobin mutants. It illustrates the basic idea and approaches of artificial hydrolase design, which provides valuable clues for rational construction of artificial hydrolase or other enzymes. The progress in rational design of artificial hydrolases not only enriches our understanding of the structure-property-reactivity-function relationship of native enzymes, but also enhances our ability to construct artificial enzymes with advanced functions.

Contents
1 Introduction
2 Mechanisms of hydrolases
3 Hydrolases containing no metal ions
3.1 Reuse of natural proteins
3.2 Reconstruction of natural proteins
4 Hydrolases containing metal ions
4.1 Molecular design based on 3-α-helix
4.2 Molecular design based on 4-α-helix
4.3 Molecular design based on zinc-finger protein
5 Hydrolases containing metal complex
5.1 Explore hydrolysis activity of heme proteins
5.2 Tune hydrolysis activity of heme proteins
6 Conclusion and outlook

Key words: protein design, hydrolases, active site, biological catalysis, mechanism

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