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Progress in Chemistry 2014, Vol. 26 Issue (08): 1427-1433 DOI: 10.7536/PC140328 Previous Articles   Next Articles

• Review •

Research of Ultrasound-Mediated Gene Delivery

Du Jianwei1, Mou Yun2, Wang Youxiang*1   

  1. 1. MOE Key Laboratory of Macromolecular Synthesis and Functionalization, Department of Polymer Science and Engineering, Zhejiang University, Hangzhou 310027, China;
    2. Department of Ultrasonography, The First Affiliated Hospital, College of Medicine, Zhejiang University, Hangzhou 310027, China
  • Received: Revised: Online: Published:
  • Supported by:

    The work was supported by the National Natural Science Foundation of China (No. 51273177, 81371571) and the Zhejiang Provincial Natural Science Foundation of China(No. LY13H180008)

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Ultrasound wave can reach the deep tissues without any harm to human body. Ultrasound contrast agent (microbubble), which can highly enhance the ultrasound backscatter intensity, is widely employed in ultrasonography. Through the cavitation effects of microbubbles, the energy of ultrasound wave will be easily accumulated at the targeted tissue and finally released with the collapse of the bubbles, which will further promote the internalization of gene/drug into the cells, leading to the high transfection efficiency. Due to its non-invasiveness and safety, recent ultrasound-mediated gene therapy shows great potential via the combination of the clinic-realizable ultrasound microbubble technology and gene vectors. The ultrasound-mediated gene therapy, can not only be used in disease diagnosis, but also promote the spatio-temporal controlled release of drug/gene by sonicating at the targeted tissue. Furthermore, microbubble-induced sonoporation can be used as the driving force to transfer gene vectors into cells by improving the permeability of different biofilm. These will together provide approach to visualization of gene delivery and efficient controlled gene expression. In this paper, the recent progress is reviewed in details from the following aspects: development of microbubbles, the mechanism and influence factors, and construction of ultrasound-mediated gene delivery system. Challenges and prospects of ultrasound-mediated gene delivery system are also discussed.

Contents
1 Introduction
2 Development of microbubbles
3 Mechanism and influence factors of ultrasound- mediated gene delivery
4 Construction of ultrasound-mediated gene delivery system
4.1 Co-administration system
4.2 Gene-loaded microbubbles
4.3 Gene vector-microbubble conjugation system
5 Challenges and prospects

Key words: ultrasound, microbubbles, gene therapy, non-viral gene vector

CLC Number: 

[1] Ginn S L, Alexander I E, Edelstein M L, Abedi M R, Wixon J. J. Gene. Med., 2013, 15: 65.
[2] 王幽香(Wang Y X), 沈家骢(Shen J C). 科学通报(Chinese Science Bulletin), 2004, 49: 819.
[3] Li W Y, Du J W, Zheng K, Zhang P, Hu Q L, Wang Y X. Chem. Commun., 2014, 50: 1579.
[4] Li J G, Cheng D, Yin T H, Chen W C, Lin Y J, Chen J F, Li R T, Shuai X T. Nanoscale, 2014, 6: 1732.
[5] Peng L, Gao Y, Xue Y N, Huang S W, Zhuo R X. Biomaterials, 2010, 31: 4467.
[6] Zhang M, Xue Y N, Liu M, Zhuo R X, Huang S W. Nanoscale Res. Lett., 2010, 5: 1804.
[7] Wang Y X, Chen P, Shen J C. Biomaterials, 2006, 27: 5292.
[8] Rychak J J, Klibanov A L. Adv. Drug. Deliv. Rev., 2014, 72: 82.
[9] Ferrara K W, Borden M A, Zhang H. Acc. Chem. Res., 2009, 42: 881.
[10] Guo X, Huang L. Acc. Chem. Res., 2011, 45: 971.
[11] Mou Y, Ye Y, Zhao X Y, Yao L, Yan L P, Sun J, Zhu Z H, Hu S J. Ultrasound Med. Biol., 2009, 35: 1638.
[12] 牟芸(Mou Y), 胡申江(Hu S J), 姚磊(Yao L), 郑哲岚(Zheng Z L). 第二届长三角超声医学论坛暨2009年浙江省超声医学学术年会论文汇编(The Second Yangtze Ultrasonic Medical BBS and Ultrasonic Medical Academic Conference Proceedings in Zhejiang Province in 2009). 2009.
[13] Greenleaf W J, Bolander M E, Sarkar G, Goldring M B, Greenleaf J F. Ultrasound Med. Biol., 1998, 24: 587.
[14] Unger E C, Hersh E, Vannan M, Matsunaga T O, McCreery T. Prog. Cardiovasc. Dis., 2001, 44: 45.
[15] 汪国忠(Wang G Z), 胡申江(Hu S J), 郑哲岚(Zheng Z L), 童紫莺(Tong Z Y), 郑霞(Zheng X), 姚宇玫(Yao Y M), 李江(Li J). 中华超声影像学杂志(Chinese Journal of Ultrasonography), 2005, 13: 857.
[16] Negishi Y, Hamano N, Tsunoda Y, Oda Y, Choijamts B, Endo-Takahashi Y, Omata D, Suzuki R, Maruyama K, Nomizu M, Emoto M, Aramaki Y. Biomaterials, 2013, 34: 501.
[17] Tlaxca J L, Rychak J J, Ernst P B, Konkalmatt P R, Shevchenko T I, Pizzaro T T, Rivera-Nieves J, Klibanov A L, Lawrence M B. J. Control. Release, 2013, 165: 216.
[18] Un K, Kawakami S, Yoshida M, Higuchi Y, Suzuki R, Maruyama K, Yamashita F, Hashida M. Hepatology, 2012, 56: 259.
[19] Un K, Kawakami S, Higuchi Y, Suzuki R, Maruyama K, Yamashita F, Hashida M. J. Control. Release, 2011, 156: 355.
[20] Omata D, Negishi Y, Hagiwara S, Yamamura S, Endo-Takahashi Y, Suzuki R, Maruyama K, Aramaki Y. J. Drug Target., 2012, 20: 355.
[21] Yan P, Chen K J, Wu J, Sun L, Sung H W, Weisel R D, Xie J, Li R K. Biomaterials, 2014, 35: 1063.
[22] Chen Z, Liang K, Xie M X, Wang X F, Lü Q, Zhang J. Mol. Biol. Rep., 2009, 36: 2059.
[23] Miller D, Pislaru S, Greenleaf J. Somat. Cell. Mol. Genet., 2002, 27: 115.
[24] Lawrie A, Brisken A F, Francis S E, Tayler D I, Chamberlain J, Crossman D C, Cumberland D C, Newman C M. Circulation, 1999, 99: 2617.
[25] Lentacker I, de Cock I, Deckers R, de Smedt S C, Moonen C T. Adv. Drug. Deliv. Rev., 2014, 72: 49.
[26] Suzuki R, Oda Y, Utoguchi N, Maruyama K. J. Control. Release, 2011, 149: 36.
[27] Mehier-Humbert S, Bettinger T, Yan F, Guy R H. J. Control. Release, 2005, 104: 213.
[28] Negishi Y, Endo Y, Fukuyama T, Suzuki R, Takizawa T, Omata D, Maruyama K, Aramaki Y. J. Control. Release, 2008, 132: 124.
[29] Un K, Kawakami S, Yoshida M, Higuchi Y, Suzuki R, Maruyama K, Yamashita F, Hashida M. Biomaterials, 2011, 32: 4659.
[30] Negishi Y, Omata D, Iijima H, Takabayashi Y, Suzuki K, Endo Y, Suzuki R, Maruyama K, Nomizu M, Aramaki Y. Mol. Pharmaceut., 2009,7: 217.
[31] Kawazu T, Hakamada K, Oda Y, Miyake J, Maruyama K, Nagasaki T. Chem. Lett., 2011, 40: 298.
[32] Duvshani-Eshet M, Machluf M. J. Control. Release, 2005, 108: 513.
[33] Lentacker I, Wang N, Vandenbroucke R E, Demeester J, de Smedt S C, Sanders N N. Mol. Pharm., 2009, 6: 457.
[34] 牟芸(Mou Y), 姚磊(Yao L), 郑哲岚(Zheng Z L), 叶炀(Ye Y). 2006年浙江省超声医学学术年会论文汇编(Ultrasonic Medical Academic Conference Proceedings in Zhejiang Province in 2006). 2006.
[35] Koshima R, Suzuki R, Oda Y, Hirata K, Nomura T, Negishi Y, Utoguchi N, Kudo N, Maruyama K. Characterisation of Gene Delivery Using Liposomal Bubbles and Ultrasound (Eds. Matsumoto Y, Crum L A, ter Haar G R). Tokyo: AIP Conference Proceedings, 2011. 330.
[36] Duvshani-Eshet M, Adam D, Machluf M. J. Control. Release, 2006, 112: 156.
[37] Nomikou N, Tiwari P, Trehan T, Gulati K, McHale A P. Acta Biomater., 2012, 8: 1273.
[38] Kinoshita M, Hynynen K. Biochem. Bioph.Res. Commun., 2007, 359: 860.
[39] Suzuki R, Takizawa T, Negishi Y, Hagisawa K, Tanaka K, Sawamura K, Utoguchi N, Nishioka T, Maruyama K. J. Control. Release, 2007, 117: 130.
[40] Alter J, Sennoga C A, Lopes D M, Eckersley R J, Wells D J. Ultrasound Med. Biol., 2009, 35: 976.
[41] Mehier-Humbert S, Yan F, Frinking P, Schneider M, Guy R H, Bettinger T. Bioconju. Chem., 2007, 18: 652.
[42] Taniyama Y, Tachibana K, Hiraoka K, Aoki M, Yamamoto S, Matsumoto K, Nakamura T, Ogihara T, Kaneda Y, Morishita R. Gene Ther., 2002, 9: 372.
[43] Li W Y, Zhang P, Zheng K, Hu Q L, Wang Y X. J. Mater. Chem. B, 2013, 1: 6418.
[44] Li W Y, Wang Y X, Chen L N, Huang Z X, Hu Q L, Ji J. Chem. Commun., 2012, 48: 10126.
[45] Qiu Y Y, Luo Y, Zhang Y L, Cui W C, Zhang D, Wu J R, Zhang J F, Tu J. J. Control. Release, 2010, 145: 40.
[46] 汪国忠(Wang G Z), 胡申江(Hu S J), 郑哲岚(Zheng Z L), 孙坚(Sun J), 郑霞(Zheng X), 朱朝晖(Zhu Z H), 李江(Li J), 姚宇玫(Yao Y M). 生物医学工程学杂志(Journal of Biomedical Engineering), 2006, 23: 856.
[47] Deshpande M C, Prausnitz M R. J. Control. Release, 2007, 118: 126.
[48] Negishi Y, Endo-Takahashi Y, Matsuki Y, Kato Y, Takagi N, Suzuki R, Maruyama K, Aramaki Y. Mol. Pharmaceut., 2012, 9: 1834.
[49] Song S, Noble M, Sun S, Chen L, Brayman A A, Miao C H. Mol. Pharmaceut., 2012, 9: 2187.
[50] Zhou Z Y, Zhang P, Ren J L, Ran H T, Zheng Y Y, Li P, Zhang Q X, Zhang M H, Wang Z G. J. Control. Release, 2013, 170: 437.
[51] Sun L, Huang C W, Wu J, Chen K J, Li S H, Weisel R D, Rakowski H, Sung H W, Li R K. Biomaterials, 2013, 34: 2107.
[52] Yin T H, Wang P, Li J G, Zheng R Q, Zheng B W, Cheng D, Li R T, Lai J Y, Shuai X T. Biomaterials, 2013, 34: 4532.
[53] Un K, Kawakami S, Suzuki R, Maruyama K, Yamashita F, Hashida M. Mol. Pharm., 2011, 8: 543.
[54] Lum A F, Borden M A, Dayton P A, Kruse D E, Simon S I, Ferrara K W. J. Control. Release, 2006, 111: 128.
[55] De Temmerman M L, Dewitte H, Vandenbroucke R E, Lucas B, Libert C, Demeester J, de Smedt S C, Lentacker I, Rejman J. Biomaterials, 2011, 32: 9128.
[56] Sirsi S R, Hernandez S L, Zielinski L, Blomback H, Koubaa A, Synder M, Homma S, Kandel J J, Yamashiro D J, Borden M A. J. Control. Release, 2012, 157: 224.
[57] Cui W, Tavri S, Benchimol M J, Itani M, Olson E S, Zhang H, Decyk M, Ramirez R G, Barback C V, Kono Y, Mattrey R F. Biomaterials, 2013, 34: 4926.
[58] Yin T H, Wang P, Zheng R Q, Zheng B W, Cheng D, Zhang X L, Shuai X T. Int. J. Nanomed., 2012, 7: 895.
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