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Progress in Chemistry 2014, Vol. 26 Issue (06): 1050-1064 DOI: 10.7536/PC131115 Previous Articles   Next Articles

• Review •

Microfluidics-Based Single-Cell Biophysical Characterization

Tang Wenlai, Xiang Nan, Huang Di, Zhang Xinjie, Gu Xingzhong, Ni Zhonghua*   

  1. Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments, Southeast University, Nanjing 211189, China
  • Received: Revised: Online: Published:
  • Supported by:

    The work was supported by the National Basic Research Program of China (No.2011CB707601), the National Natural Science Foundation of China (No.91023024, 51375089), the Specialized Research Fund for the Doctoral Program of Higher Education (No.20110092110003) and the Natural Science Foundation of Jiangsu Province (No.BK2011336)

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Single-cell biophysical characterization has been widely employed for demonstrating the physiological activity and status of individual cells, or revealing the heterogeneity among various populations. It also plays an important role in the studies on cellular differentiation and pathology, as well as early clinical diagnosis and treatment. Compared to conventional biochemical schemes, the feature of scale compatibility between cell and microchannel makes microfluidics more suitable for precise microenvironment control, high-throughput manipulation, and multi-parameter label-free detection of individual cells. Therefore, microfluidics has become an important platform for single-cell characterization and analysis. This review covers the recent advances in microfluidics for characterizing the single-cell biophysical properties, and then focuses on the discussion of specific advantages, such as single-cell resolution level and high-throughput feature, offered by microfluidics. Finally, the challenges and future directions concerning the application of this scheme in clinical practices are also discussed, and a novel single-cell microdevice for multi-parameter characterization is proposed.

Contents
1 Introduction
2 Density(Mass) characterization
3 Electrical characterization
3.1 Microfluidic Coulter counter
3.2 Microfluidic impedance cytometer
4 Mechanical characterization
4.1 Optical stretcher
4.2 Electroporation-induced deformation
4.3 Structure-induced deformation
4.4 Fluid-induced deformation
5 Multi-parameter biophysical characterization
6 Conclusion and outlook

Key words: single-cell, biophysical properties, microfluidics, high-throughput, multi-parameter

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