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Progress in Chemistry 2013, Vol. 25 Issue (07): 1198-1207 DOI: 10.7536/PC121161 Previous Articles   Next Articles

Special Issue: 酶化学

• Review •

Preparation and Application of Immobilized Enzyme Micro-Reactor

Shen Gangyi1*, Yu Wanting1, Liu Meirong2, Cui Xun1,3*   

  1. 1. Institute of Chinese Minority Traditional Medicine, Minzu University of China, Beijing 100081, China;
    2. Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China;
    3. Department of Oriental Pharmacy, Woosuk University,Wanju-gun,Jeonbuk 565-701,South Korea
  • Received: Revised: Online: Published:
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As one kind of new biochemical reaction device, immobilized enzyme micro-reactor is the combination of biomolecule immobilizing technique and modern micro-reaction method. In view of its advantages in efficiency, economy and addressable recognition specially, micro-reactor plays a significant role in the research of life science, such as proteomics, screening of enzyme inhibitors, biocatalysis and so on. With the development of immobilizing materials and fabrication methods, the performance of enzyme micro-reactor has been improved greatly, and enzyme micro-reactor has been applied to many research fields. This article focuses on the preparation methods and the applications of immobilized enzyme micro-reactor for the past few years. The advantages and shortcomings of the current state-of-the-art preparation methods are particularly discussed. In addition, the prospects of its future study are outlined. Contents
1 Introduction
2 Preparation of immobilized enzyme micro-reactor
2.1 Convalent bonding
2.2 Physical adsorption
2.3 Encapsulation
2.4 Metal-ion chelated adsorption
2.5 Biological binding
3 Applications
4 Outlook

Key words: immobilized enzyme micro-reactor, preparation, proteomics, screening of enzyme inhibitors, biocatalysis

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