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化学进展 2015, Vol. 27 Issue (12): 1799-1807 DOI: 10.7536/PC150636 前一篇   后一篇

• 综述与评论 •

基于共轭聚合物的核酸生物传感器的应用

马昀, 周妍, 杜文琦, 缪智辉, 祁争健*   

  1. 东南大学化学化工学院 南京 211189
  • 收稿日期:2015-06-01 修回日期:2015-08-01 出版日期:2015-12-15 发布日期:2015-09-17
  • 通讯作者: 祁争健 E-mail:qizhengjian@seu.edu.cn
  • 基金资助:
    江苏省科技成果转化专项基金(No.BA2014123)和国家重大科学仪器设备开发专项(No.2014YQ060773)资助
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导出 被引次数 ( 2 | 2 )

The Application of DNA Biosensor Based on Conjugated Polymers

Ma Yun, Zhou Yan, Du Wenqi, Miao Zhihui, Qi Zhengjian*   

  1. School of Chemistry and Chemical Engineering, Southeast University, Nanjing 211189, China
  • Received:2015-06-01 Revised:2015-08-01 Online:2015-12-15 Published:2015-09-17
  • Supported by:
    The work was supported by the Jiangsu Province Transformation of Scientific and Technological Achievements Program (No. BA2014123) and the National Major Scientific Instruments and Equipment Development Projects(No. 2014YQ060773).
共轭聚合物的π电子体系及共轭离域结构,使其具有良好的发光性能。聚合物链可充当“分子导线”,能够成倍放大光学信号,从而有效提高检测灵敏度。而核酸适体(aptamer)在特异性、与靶物质亲合力、信号传导方面比其他识别元件具有更大的优势,因此共轭聚合物的核酸生物传感器在生物检测方面得到了迅速发展。本文主要总结了近年来共轭聚合物的核酸生物传感器在生物检测方面的应用,并进一步对该类型传感器的发展趋势作出了展望。
关键词： 共轭聚合物, 核酸适体, 生物检测, 荧光共振能量转移, 构象变化
Conjugated polymers, with π electron systems and highly delocalized conjugated structures, exhibit excellent luminescence properties. The polymer chains can work as molecular wire, which will lead to the amplification of optical signals and thus improve the detecting sensitivity. Aptamer has advantages in specificity, affinity with targets and signal transmission, hence, nucleic acid biosensors based on conjugated polymers have witnessed a rapid development in bio-detection. The applications of nucleic acid biosensors based on conjugated polymers in recent years are summarized. Finally, an outlook of the developing trend for these sensors is given.

Contents
1 Introduction
2 The sensing mechanism of fluorescent sensor
3 FRET
3.1 Detection of complementary DNA
3.2 Real-time monitoring of DNA hybridization
3.3 Real-time monitoring of DNA structure
3.4 Detection of protein and the activity of enzyme
3.5 Detection of specific gene
4 Aggregation and conformation change
4.1 Detection of complementary DNA
4.2 Real-time monitoring of DNA hybridization
4.3 Real-time monitoring of DNA structure
4.4 Detection of enzyme
4.5 Detection of specific DNA sequence
4.6 Detection of metal ion
5 Superquenching
6 Conclusion

Key words: conjugated polymer, aptamer, biological detection, FRET, conformation change

中图分类号: 

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