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化学进展 2013, Vol. 25 Issue (10): 1631-1641 DOI: 10.7536/PC130115 前一篇   后一篇

• 综述与评论 •

金纳米颗粒的细胞毒性和促细胞生长作用

江龙, 王清叶, 崔文娟   

  1. 北京分子科学国家实验室 中国科学院化学研究所 胶体、界面与化学热力学科学院重点实验室 北京100190
  • 收稿日期:2013-03-01 修回日期:2013-06-01 出版日期:2013-11-12 发布日期:2013-07-18
  • 通讯作者: 江龙 E-mail:jiangl@iccas.ac.cn
  • 基金资助:

    国家自然科学基金项目(No.91127012,21161130521,20933007,21021003)资助

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Influence of Gold Nanoparticles on the Cytotoxity and Cell Growth

Jiang Long, Wang Qingye, Cui Wenjuan   

  1. Beijing National Laboratory for Molecular Sciences, Key Laboratory of Colloid, Interface and Chemical Thermodynamics, Institute of Chemistry, CAS, Beijing 100190, China
  • Received:2013-03-01 Revised:2013-06-01 Online:2013-11-12 Published:2013-07-18

虽然对于纳米金颗粒(AuNPs)和细胞相互作用的研究有着大量的报道,但是其结果常常是互相矛盾的。对其机理的研究更是十分的贫乏。这些研究中的一个核心问题,即AuNPs对生物体或细胞是有毒的还是有利于其生长的。虽然AuNPs在生物体内的作用远比体外的简单细胞反应要复杂得多,涉及到与生物体中多种组分的作用,从而引起极为不同的免疫反应、代谢机理等。但是在体外进行纳米颗粒的毒性和促进细胞生长相互作用的微观研究仍将为AuNPs在体内的作用提供十分重要的理论依据。本综述主要从AuNPs尺寸、表面电荷、颗粒聚集状态等方面介绍近年来关于AuNPs的细胞毒性和促细胞生长的体外研究结果,并对其作用机理进行初步的探讨。

关键词： 纳米金颗粒聚集体, 细胞毒性和促细胞生长, 细胞胞吞作用, 细胞毒性动力学

The cytotoxicity of gold nanoparticles(AuNPs) and the effects on cell growth have been reported trememdously, however the experiment results are often contradicted. The studies on the underlying mechanism are even rarely reported.A core problem in these researches is whether the AuNPs are toxic or benefit to the cell growth. It is understandable that the reactions of AuNPs in human body is much more complicated than those observed in vitro. Multiple components are involved in the human body, causing different immune responses and metabolism outputs. Nevertheless, microscopic investigation outside the human body still is a very important method to provide evidences for the mechanism of the role of AuNPs playing in the body.This review mainly introduces the results of the cytotoxicity of AuNPs and the effects on cell growth in vitro in terms of the size, surface charge and component, and the aggregation of AuNPs. The possible machanism involving in the nanoparticle effect has been discussed.

Contents
1 Introduction
2 Influence of size and surface property of gold nanoparticles (AuNPs) on the cytotoxicity
2.1 Influence of AuNPs size on the cytotoxicity
2.2 Influence of surface charge and protective layer of AuNPs on the cytotoxicity
2.3 Endocytosis
2.4 Influence of cell growth medium on the AuNPs aggregation state
3 The cytotoxicity assessment system and the mechanism of cytotoxicity of AuNPs
3.1 Assessment methods for cytotoxicity
3.2 Kinetic study of cytotoxicity process: Influence of AuNPs concentration and interaction time
3.3 Possible mechanism of cytotoxicity caused by AuNPs
4 Influence on cell growth caused by AuNPs
5 Conclusion

Key words: aggregation of gold nanoparticles, cytotoxity and cell growth, endocytosis, dynamic of cytotoxity

中图分类号: 

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