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化学进展 2008, Vol. 20 Issue (11): 1779-1787 前一篇   后一篇

• 综述与评论 •

分子阵列研究进展

刘少斌 张庆林 吴祖泽   

  1. 天津大学化工学院制药工程系;军事医学科学院放射与辐射医学研究所 军事医学科学院放射与辐射研究所
  • 收稿日期:2007-12-20 修回日期:2008-02-28 出版日期:2008-11-24 发布日期:2008-11-25
  • 通讯作者: 刘少斌

Progress in Small Molecule Microarrays

Liu Shaobin Qinglin Zhang Wu Zuze   

  • Received:2007-12-20 Revised:2008-02-28 Online:2008-11-24 Published:2008-11-25
  • Contact: Liu Shaobin
小分子化合物可以调节生物学过程,是研究活性生物大分子(特别是蛋白质)以及药物的重要工具,而高通量筛选是发现活性分子的重要方法。分子阵列是近年来新出现的一种高通量筛选技术,上面含有成千上万种组合合成的化合物以及天然产物,可以用于发现新的先导化合物,以及筛选已有的先导化合物。现在分子阵列已经成功应用于蛋白分析、先导化合物的发现等许多领域。本文综述了近年来分子阵列的构建过程,原位合成、非原位合成等各种固定化策略以及荧光免疫检测,表面等离子体共振成像技术等检测手段,并介绍了化学分子印刷阵列方法,最后总结了分子阵列的应用,并对分子阵列在我国中药发展等有方面将起到的潜在作用作了展望。
Small molecules that regulate biological processes serve as valuable tools in studies of the functions of biomolecules, especially proteins, as well as in the development of drugs. An important component of efforts that target the discovery of bioactive molecules is high-throughput screening (HTS). Newly emerging platform of high-throughput screening technology are small molecule microarrays. These arrays contain thousands of drug-like small molecules from parallel combinatorial synthesis and nature products. They enable the identification of new, and the screening of existing, lead molecules. They have already been successfully applied in important areas ranging from protein profiling to the discovery of therapeutic leads. Recent interesting developments towards improved immobilization strategies (such as in situ synthesis, non-in situ synthesis et al) and test methods (fluorescence immunodetection, surface plasmon resonance imaging technology) together with applications in academia and industry was described herein. The microarrays prepared by chemical lithography are introduced, too. As a rapidly maturing technology, SMMs will play an important role on the development of Chinese traditional drugs.

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分子阵列研究进展