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化学进展 2016, Vol. 28 Issue (5): 697-710 DOI: 10.7536/PC151118 前一篇   后一篇

• 综述与评论 •

微悬臂生物传感器

戴莹萍1, 嵇正平1, 王赪胤1*, 胡效亚1*, 汪国秀2   

  1. 1. 扬州大学化学化工学院 扬州 225002;
    2. 悉尼科技大学数学与物理科学学院 NSW 2007 澳大利亚
  • 收稿日期:2015-11-01 修回日期:2016-01-01 出版日期:2016-05-15 发布日期:2016-03-25
  • 通讯作者: 王赪胤, 胡效亚 E-mail:wangcy@yzu.edu.cn;xyhu@yzu.edu.cn
  • 基金资助:
    国家自然科学基金项目(No.21375116)、国家高技术研究发展计划(863)项目(No.2009AA03Z331)和江苏省大型科学仪器设备共享服务平台分析测试技术及方法研究课题(BZ201409)资助
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导出 被引次数 ( 5 | 1 )

Microcantilever Biosensors

Dai Yingping1, Ji Zhengping1, Wang Chengyin1*, Hu Xiaoya1*, Wang Guoxiu2   

  1. 1. College of Chemistry and Engineering, Yangzhou University, Yangzhou 225002;
    2. School of Mathematical and Physical Sciences, University of Technology Sydney, NSW 2007, Australia
  • Received:2015-11-01 Revised:2016-01-01 Online:2016-05-15 Published:2016-03-25
  • Supported by:
    The work was supported by the National Natural Science Foundation of China (No. 21375116), the National High Teehnology Research and Development Program of China (No.2009AA03Z331) and the Research Program on Analytical Methods and Techniques on the Shared Platform of Large-Scale Instruments and Equipment in Jiangsu Province(BZ201409).
微悬臂生物传感器是基于原子力显微镜和生物传感器发展而来,具有无需标记、快速、实时、灵敏度高等优点,已广泛运用于生物医学、环境监测、医药、食品及军事等多个领域。微悬臂生物传感器已成为当前研究热点。本文对近年来微悬臂生物传感器的研究工作进行了综述,总结了微悬臂传感器中微悬臂梁的结构、工作模式、激励方法和检测方法。国内有关微悬臂传感器的综述多是着重于介绍微悬臂传感器的应用进展,而缺少对微悬臂传感器的检测方法或读出方式做全面而系统的介绍,本文不仅全面且系统地对微悬臂传感器的8种检测方法进行了详细的介绍和分析,还介绍了微悬臂梁的多种典型尺寸、外观和微悬臂传感器工作模式中的"热模式"。针对微悬臂传感器实现特定检测及提高灵敏度的目的,本文总结了微悬臂传感器的表面修饰方法,不仅介绍了国内外微悬臂生物传感器最新的应用动态,还介绍了一种具有自驱动自传感功能的新型微悬臂传感器,并对微悬臂生物传感器的应用前景作了探讨。
关键词： 微悬臂, 生物传感器, 原子力显微镜, 检测, 应用
Microcantilever biosensors based on atomic force microscope have the advantages of label-free, rapid, real-time, and high sensitivity detection features. Therefore, microcantilever biosensors can be applied in the fields of biomedicine, environmental monitoring, food production and military defenses. Microcantilever biosensors have become the focus of scientific research. In this paper, we review the working principle, excitation method and detection mechanism of microcantilever biosensors. Preview reviews about the microcantilever biosensors mainly focuse on the progress of applications, but lack of comprehensive and systematic introduction on detection methods. Herein, we not only systematically analyze eight detection methods, but also introduce several typical sizes, appearance and "heat mode" of microcantilever biosensors. So far, these have not been reported in literatures. In order to realize specific detection and improve sensitivity of microcantilever sensors, we summarize the surface modification methods of microcantilever sensors and review the latest applications of microcantilever biosensors. Furthermore, we introduce a new kind of self-actuating and self-sensing microcantilever biosensors. The prospective of microcantilever biosensors is also discussed in this paper.

Contents
1 Introduction
2 Microcantilever biosensors
3 The brief introduction of microcantilever beam
4 Microcantilever working mode
4.1 Dynamic working mode
4.2 Static working mode
4.3 Heat mode
5 Excitation methods of the microcantilever biosensors
6 Detection methods of the microcantilever biosensors
6.1 Optical lever
6.2 Optical interferometry
6.3 Piezoresistive method
6.4 Piezoelectric method
6.5 Capacitance method
6.6 Electron tunneling method
6.7 Other methods
7 Surface biofunctionalization
8 Application of the microcantilever biosensors
8.1 DNA hybridization detection
8.2 Pathogens detection
8.3 Protein detection
8.4 Small-molecular and drug testing
8.5 Biological warfare agent detection
8.6 Self-actuating and self-sensing microcantilever biosensors
9 Conclusion and outlook

Key words: microcantilever, biosensor, atomic force microscope, detection, application

中图分类号: 

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摘要

微悬臂生物传感器