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化学进展 2010, Vol. 22 Issue (01): 148-152 前一篇   后一篇

• 综述与评论 •

应用前沿亲和色谱研究分子之间相互作用及其应用

卢时湧1,2;吴章桂2;叶伟东2;吴国锋2;潘一斌2;钱俊青3*   

  1. （1. 浙江工业大学生物与环境学院    杭州 310014；2. 浙江医药股份有限公司新昌制药厂   绍兴 310025；3. 浙江工业大学药学院   杭州 310014）
  • 收稿日期:2009-02-16 修回日期:2009-05-05 出版日期:2010-01-24 发布日期:2010-01-07
  • 通讯作者: 钱俊青 E-mail:qjq@zjut.edu.cn
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Application of Frontal Affinity Chromatography to Studies on Interactions of Molecules

Lu Shiyong1,2;  Wu Zhanggui2; Ye Weidong2;  Wu Guofeng2;  Pan Yibin2;  Qian Junqing3*   

  1. (1. College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China; 2. Zhejiang Medicine Co., Ltd., Shaoxing 310025,China; 3. College of Pharmacy, Zhejiang University of Technology, Hangzhou 310014, China )
  • Received:2009-02-16 Revised:2009-05-05 Online:2010-01-24 Published:2010-01-07
  • Contact: Qian Junqing E-mail:qjq@zjut.edu.cn

分子间的相互作用是一切生物或化学变化的基础。前沿亲和色谱作为通用性的研究分子间相互作用力的工具，利用它可得到分子之间相应的解吸常数Kd，并可对各种配体的亲和力大小进行排序。本文介绍了前沿亲和色谱的工作原理、前沿亲和色谱柱的构建，以及在药物筛选，生物相关领域的应用。与其他研究分子间相互作用的技术作了相应的比较。

关键词： 前沿亲和色谱, 分子间相互作用, 药物筛选

Molecule interaction is important to the biological process and chemical reaction. Frontal affinity chromatography is a universal tool enabling to study the direct molecule interaction, to rank ligands and determine Kd. The review summarizeS the frontal affinity chromatography theory, the applications and the difference with other technologies that can study the direct molecule interaction.

Contents
1 Introduction
2 Theory and components of FAC
2.1 Theory of FAC
2.2 Measurement of Kd
2.3 Components of FAC
2.4 Modes of FAC
3 Applications of FAC
3.1 Analyzing protein purity
3.2 Characterizing molecular interactions
3.3 Differentiating between multiple binding sites of target molecules
3.4 Screening drugs
4 Comparison of techniques based on direct binding
5 Outlook

Key words: frontal affinity chromatography (FAC), intermolecular interaction, drug screening

中图分类号: 

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[ 1 ] 　Cai Z, GreeneM I, Berezov A. Methods, 2008, 46: 39—46
[ 2 ] 　Fabian M A, BiggsW H Ⅲ, Treiber D K, et al. Nature Biotechnology, 2005, 23: 329—336
[ 3 ] 　ArkinM R, Wells J A. Nature Reviews Drug Discovery, 2004,3: 301—317
[ 4 ] 　Malmqvist M, Karlsson R. Current Opinion in Chemical Biology, 1997, 1: 378—383
[ 5 ] 　Yamada KM. Annual Review of Biochemistry, 1983, 52:761—799
[ 6 ] 　Kasai K, Ishii S. J. Biochem. , 1975, 77: 261—264
[ 7 ] 　Hage D S, Tweed S A. J. Chromatogr. B, 1997, 699:499—525
[ 8 ] 　Hage D S. J. Chromatogr. B, 2002, 768: 3—30
[ 9 ] 　HaoW, Wang J. J. Chromatogr. A, 2005, 1063: 47—56
[ 10 ] 　Bertucci C, BartoliniM, Gotti R, et al. J. Chromatogr. B,2003, 797: 111—129
[ 11 ] 　Moaddel R, Wainer IW. Journal of Pharmaceutical and Biomedical Analysis, 2007, 43: 399—406
[ 12 ] 　Kasai K I, Oda Y. J. Chromatogr. , 1986, 376: 33—47
[ 13 ] 　Slon-Usakiewicz J J, Ng W, Dai J R, et al. Drug Discovery Today, 2005, 10: 409—416
[ 14 ] 　Moaddel R, Wainer IW. Anal. Chim. Acta, 2006, 564: 97—105
[ 15 ] 　Cheng Y, Ho E, Subramanyam B, et al. J. Chromatogr. B,2004, 809: 67—73
[ 16 ] 　Beigi F, Chakir K, Xiao R P, Wainer I W. Anal. Chem. ,2004, 76: 7187—7193
[ 17 ] 　Besanger T R, Hodgson R J, Guillon D, et al. Anal. Chim.Acta, 2006, 561: 107—118
[ 18 ] 　Puerta A, Vidal-Madjar C, Jaulmes A, et al. J. Chromatogr.A, 2006, 1119: 34—42
[ 19 ] 　Su X, Qin F, Kong L, et al. J. Chromatogr. B, 2007, 845:174—179
[ 20 ] 　Moaddel R, Bullock P L, Wainer I W. J. Chromatogr. B,2004, 799: 255—263
[ 21 ] 　Gottschalk I, Li YM, Lundahl P. J. Chromatogr. B, 2000,739: 55—62
[ 22 ] 　Tetala K K R, Chen B, Visser G M, et al. J. Biochem. Biophys. Methods, 2007, 70: 63—69
[ 23 ] 　Slon-Usakiewicz J J, Dai J R, Ng W, et al. Anal. Chem. ,2005, 77: 1268—1274
[ 24 ] 　Gupta R, Chaudhury N K. Biosensors and Bioelectronics,2007, 22: 2387—2399
[ 25 ] 　Hodgson R J, BrookM A, Brennan J D. Anal. Chem. , 2005,77: 404—4412
[ 26 ] 　LiW, FriesD P, Malik A. Journal of Chromatography A,2004, 1044: 23—52
[ 27 ] 　Cichna-MarklM. Journal of Chromatography A, 2006, 1124:167—180
[ 28 ] 　J inW, Brennan J D. Analytica Chimica Acta, 2002, 461:1—36
[ 29 ] 　Zacharis C K , Kalaitzantonakis E A, Podgornik A, et al. J.Chromatogr. A, 2007, 1144: 126—134
[ 30 ] 　Tetala K K R, Chen B, Visser G M, et al. J. Biochem. Biophys. Methods, 2007, 70: 63—69
[ 31 ] 　Fort S, Kim H S, Hindsgaul O. J. Org. Chem. , 2006, 71:7146—7154
[ 32 ] 　de BoerA R, Lingeman H, Niessen W M A, et al. Trends in Analytical Chemistry, 2007, 26 (9) : 867—883
[ 33 ] 　Deng G, Sanyal G. Journal of Pharmaceutical and Biomedical Analysis, 2006, 40: 528—538
[ 34 ] 　Lundqvist A, Brekkan E, Lagerquist C, et al. Mater. Sci. Eng.C, 1997, 4: 221—226
[ 35 ] 　Slon-Usakiewicz J J, NgW, Foster J E, et al. J. Med. Chem. ,2004, 47: 5094—5100
[ 36 ] 　Haneskog L, Zeng CM, Lundqvist A, et al. Biochimica et Biophysica Acta, 1998, 1371: 1—4
[ 37 ] 　Hirabayashi J, Arata Y, Kasai K. J. Chromatogr. A, 2000,890: 261—271
[ 38 ] 　Lin S, Drake L R, Rayson G D. Anal. Chem. , 1996, 68:4087—4093
[ 39 ] 　Slon-Usakiewicz J J, Dai J R, Ng W, et al. Anal. Chem. ,2005, 77: 1268—1274
[ 40 ] 　Zacharis C K, Kalaitzantonakis E A, Podgornik A, et al. J.Chromatogr. A, 2007, 1144: 126—134
[ 41 ] 　Boguslavsky, J. Drug Discov. Dev. , 2004, 7: 37—40
[ 42 ] 　Schriemer D C, Bundle D R, Li L, et al. Angew. Chem. Int.Ed. , 1998, 37: 3383—3387
[ 43 ] 　Ng E SM, Yang F, Kameyama A, et al. Anal. Chem. , 2005,77: 6125—6133
[ 44 ] 　Toledo-Sherman L, Deretey E, Slon-Usakiewicz J J, et al. J.Med. Chem. , 2005, 48: 3221—3230
[ 45 ] 　Zhang B, Palcic M M, Schriemer D C, et al. Anal. Biochem. ,2001, 299: 173—182
[ 46 ] 　Zhu L, Chen L, Luo H, et al. Anal. Chem. , 2003, 75:6388—6393
[ 47 ] 　Luo H, Chen L, Li Z, et al. Anal. Chem. , 2003, 75: 3994—3998
[ 48 ] 　Zhu L L, Xu X J. Acta Phys. Chim. Sin. , 2003, 19:385—388
[ 49 ] 　Rich R L, Myszka D G. . Anal. Biochem. , 2007, 361: 1—6
[ 50 ] 　Myszka D G. Anal. Biochem. , 2004, 329: 316—323
[ 51 ] 　CooperM A. Curr. Op in. Pharmacol. , 2003, 3: 557—562
[ 52 ] 　Mattei B, Borch J, Roep storff P. Anal. Chem. , 2004, 1:19A—25A
[ 53 ] 　Roberts G C K. Drug Discovery Today, 2000, 5 (6) : 230—240
[ 54 ] 　Villar H O, Yan J, Hansen M R. Curr. Op in. Chem. Biol. ,2004, 8: 387—391
[ 55 ] 　Coles M, Heller M, Kessler H. Drug Discovery Today, 2003, 8(17) : 803—810
[ 56 ] 　Annis D A, Nazef N, Chuang C C, et al. J. Am. Chem. Soc. ,2004, 126: 15495—15503
[ 57 ] 　Schriemer D C. Anal. Chem. , 2004, 76: 440A—48A
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