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化学进展 2009, Vol. 21 Issue (10): 2009-2016 前一篇   后一篇

• 综述与评论 •

氢酶分子聚集体薄膜的组装及其在催化产氢中的应用*

钱东金**;刘安   

  1. (复旦大学化学系  上海 200433)
  • 收稿日期:2008-10-27 修回日期:2008-12-09 出版日期:2009-10-24 发布日期:2009-10-09
  • 通讯作者: 钱东金 E-mail:djqian@fudan.edu.cn
  • 基金资助:

    国家自然科学基金

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Fabrication of Hydrogenase Molecular Assemblies for BioHydrogen Production

Qian Dongjin**; Liu an   

  1. (Department of Chemistry, Fudan University, Shanghai 200433, China)
  • Received:2008-10-27 Revised:2008-12-09 Online:2009-10-24 Published:2009-10-09
  • Contact: Qian Dongjin E-mail:djqian@fudan.edu.cn
  • Supported by:

    National Natural Science Foundation of China

氢气作为一种可再生和零排放的清洁能源，在全球能源和环境双重危机的今天倍受各国政府、企业和研究人员的关注。自然界中存在于藻类和细菌中的氢酶是高效的催化氢气氧化和质子还原的氧化还原酶，在生物产氢和能量转换过程中发挥着重要的作用。近年来涌现出了许多基于氢酶及其模型化合物的仿生产氢和生物燃料电池方面的研究工作。本文综述了氢酶及其分子聚集体薄膜在电极表面的组装技术，如吸附法、自组装法、Langmuir-Blodgett法和溶胶－凝胶法等，并讨论了分子聚集体薄膜中氢酶的结构、生物活性、电化学性质及其在催化产氢方面的应用。

关键词： 氢酶, 分子聚集体, 界面组装, 电化学, 生物产氢

Hydrogen is one of the centerpieces of a sustainable, carbon-free energy supply, which has been attracted growing attention because of the rapid consumption of fossil fuel and unacceptable environmental problems such as the greenhouse effect. In nature, some green algae can produce hydrogen after incubation under anaerobic conditions, during which hydrogenase is synthesized and activated. The hydrogenase can reversely catalyze oxidation of hydrogen gas and reduction of protons, thus attracted much attention in the biohydrogen production and biofuel cells. This paper reviews recent developments in the design and assembly of hydrogenase-modified electrodes, wherein the enzyme was immobilized by physical adsorption, self-assembly, sol-gel and Langmuir-Blodgett methods. Electrochemical properties of hydrogenase in the molecular assemblies and biohydrogen production are discussed.

Contents
1 Introduction
2 Physical adsorption
3 Coadsorption
3.1 Polymer-hydrogenase coadsorption
3.2 Clay-hydrogenase-polyviologen coadsorption
3.3 Carbon nanotubes-hydrogenase coadsorption
3.4 Coadsorption of self-assembled monolayers of porphyrin/viologen and hydrogenase
4 Langmuir-Blodgett method
5 Covalently bonding method
6 Sol-gel method
7 Conclusion

Key words: hydrogenase, molecular aggregates, interfacial assembly, electrochemistry, biohydrogen production

中图分类号: 

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