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化学进展 2009, Vol. 21 Issue (09): 1895-1904 前一篇   后一篇

• 综述与评论 •

基于微流控芯片电泳的生物分子间相互作用研究*

姜萍|屈锋|谭信|李勤|耿利娜|邓玉林   

  1. （北京理工大学生命科学与技术学院 |北京100081）
  • 收稿日期:2008-09-27 修回日期:2008-12-05 出版日期:2009-09-24 发布日期:2009-09-15
  • 通讯作者: 耿利娜;邓玉林 E-mail:lngeng@hotmail.com;deng@bit.edu.cn
  • 基金资助:

    中德中心（GZ364）;国家自然科学基金

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The Application of Microfluidic Chip Electrophoresis in Biomolecular Interaction Research

JiangPing; |Qu Feng; |Tan Xin; |Li Qin; |Geng Lina** |Deng Yulin**   

  1. (School of Life Science and Technology| Beijing Institute of Technology| Beijing 100081, China)
  • Received:2008-09-27 Revised:2008-12-05 Online:2009-09-24 Published:2009-09-15
  • Contact: Geng Lina;Deng Yulin E-mail:lngeng@hotmail.com;deng@bit.edu.cn

用合适的手段表征生物分子的相互作用对于深刻理解生命过程的本质以及进行医药开发都具有重要意义。将微流控芯片和毛细管电泳相结合的微流控芯片电泳技术具有快速、高效、高通量、样品用量少和易于整合等诸多优势。本文对近年来进行生物分子间相互作用结合常数测定以及结合动力学研究的微流控芯片电泳分离模式、分析方法和芯片检测方法分别做了介绍；简单对比了微流控芯片技术和微阵列生物芯片生物分子间相互作用研究技术；最后分析了微流控芯片技术目前的不足，并对其未来的发展进行了展望。

关键词： 微流控芯片电泳, 生物分子相互作用, 分离模式, 检测方法

It is of great significance to deeply understand the life progress and promote medicine developments using appropriate techniques to characterize interaction between biomolecules. Microfluidic chip electrophoresis technique which combines microfluidic chip with capillary electrophoresis possesses many advantages including rapid, high performance, high throughput, low sample consumption and easy of integration, etc. It has already demonstrated great potential just in the early stage of application. In this paper, the separation mode, analysis methods and detection techniques in chip-CE biological binding constant and binding mechanism are reviewed respectively. The chip-CE is also simply compared with micro-array chip biomolecular interaction analysis. Finally, the development prospects and present situations in chip-CE are analyzed.

Contents
1 Introduction
2 Different microfluidic chip electrophoresis separation mode for biointeraction
2.1 Microfluidic chip zone migration electrophoresis
2.2 Microfluidic chip sieving electrophoresis
2.3 Microfluidic chip isoelectric focusing
2.4 Microchip electrochromatography
2.5 Microchip micellar electrokinetic chromatography
2.6 Microchip affinity electrophoresis
2.7 Microchip multichannel electrophoresis
3 Estimation of binding constants
4 Detection techniques of microfluidic chip electrophoresis
4.1 Laser-induced fluorescence detection
4.2 Electrochemical detection
4.3 Electrochemical luminescence detection
4.4 Mass spectrometry
4.5 UV absorbance detection
4.6 Surface plasmon resonance
5 Other techniques based on chip involved in biomolecular research
6 Conclusion and perspective

Key words: microfluidic chip electrophoresis, biomolecular interaction, separation mode, detection techniques

中图分类号: 

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