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化学进展 2019, Vol. 31 Issue (7): 996-1006 DOI: 10.7536/PC190103 前一篇   后一篇

• •

用于N-糖肽/糖蛋白分离富集的新型材料

梁阿新1, 汤波1, 孙立权1, 张鑫1,2, 侯慧鹏1, 罗爱芹1,*()   

  1. 1.北京理工大学生命学院 北京 100081
    2.南阳师范学院生命科学与技术学院 南阳 473061
  • 收稿日期:2019-01-04 出版日期:2019-07-15 发布日期:2019-04-26
  • 通讯作者: 罗爱芹
  • 作者简介:
    * E-mail:
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New Materials for the Separation and Enrichment of N-Glycopeptides/Glycoproteins

Axin Liang1, Bo Tang1, Liquan Sun1, Xin Zhang1,2, Huipeng Hou1, Aiqin Luo1,*()   

  1. 1.School of Life Science, Beijing Institute of Technology, Beijing 100081, China
    2.School of Life Science and Technology, Nanyang Normal University, Nanyang 473061, China
  • Received:2019-01-04 Online:2019-07-15 Published:2019-04-26
  • Contact: Aiqin Luo

蛋白质糖基化在调节各种复杂的生物过程中,如分子识别、免疫应答和蛋白质折叠等起着至关重要的作用。由于糖肽/糖蛋白在复杂生物样品或临床样品中丰度较低,进行糖蛋白组学分析前往往需要进行目标蛋白的分离富集。研究开发具有高效糖蛋白分离富集能力的新型材料对糖蛋白/糖肽的研究具有重要意义。近年来报道了许多新型糖蛋白分离富集材料,如有机高分子材料、生物基材料、新型有机骨架材料和新型功能复合材料等。这些材料因其结构、生物相容性和理化性质等特点,从不同层面推动了糖蛋白分离富集技术的发展。本文就目前国内外有关糖肽/糖蛋白分离富集的新型材料进行了总结和讨论,并对其未来发展提出展望。

关键词： 蛋白质糖基化, 糖蛋白, 糖肽, 分离富集, 新型材料

Protein glycosylation plays a crucial role in regulating various complex biological processes, such as molecular recognition, immune response, and protein folding. Since glycopeptides/glycoproteins are less abundant in complex biological samples or clinical samples, separation and enrichment of target proteins are often required before glycoproteomics analysis. Research and development of novel materials with high-efficiency glycoprotein separation and enrichment ability are of great significance for the study of glycoprotein/glycopeptide. In recent years, many new glycoprotein separation and enrichment materials have been reported, such as organic polymer materials, bio-based materials, new organic framework materials and new functional composite materials. Due to their structure, biocompatibility and physical and chemical properties, these materials have promoted the development of glycoprotein separation and enrichment technology from different levels. In this paper, the new materials related to the separation and enrichment of glycopeptide/glycoprotein at home and abroad are summarized and discussed, and the future development is proposed.

Key words: protein glycosylation, glycoprotein, glycopeptide, separation and enrichment, new material
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图1 (a)BTZ、(b)BZ-PDI及(c)POP-1的合成示意图[31]
Fig. 1 Fabrication routes of(a) benzene-1,3,5-tricarbohydrazide(BTZ),(b) 4,4'-(1,4-phenylene)-bis(1-phenylsemicarbazide)(BZ-PDI) and(c) polyacylsemicarbazide material(POP-1)[31]
图2 CS@PGMA@IDA的合成示意图[39]
Fig. 2 Synthesis of CS@PGMA@IDA[39]
图3 基于TpPa-1的糖基化检测方法示意图[52]
Fig. 3 The procedure for enrichment and detection of the glycopeptides based on TpPa-1[52]
图4 SBA-15-SO3H合成示意图[57]
Fig. 4 Synthesis of SBA-15-SO3H[57]
图5 Cys-Mal@SiO2制备流程图[58]
Fig. 5 Procedure of the preparation of Cys-Mal@SiO2[58]
图6 Poly-Pro@SiO2合成路线图[59]
Fig. 6 Synthesis of Poly-Pro@SiO2[59]
图7 Fe3O4@MPS@PMAC 纳米粒子合成示意图[66]
Fig. 7 Schematic illustration of the synthetic procedure of Fe3O4@MPS@PMAC nanoparticles[66]
图8 Fe3O4@PMAH 核-壳型磁性纳米复合材料合成示意图[68]
Fig. 8 Synthetic procedure for preparation of Fe3O4@PMAH core-shell magnetic nanocomposites[68]
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