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化学进展 2019, Vol. 31 Issue (8): 1129-1135 DOI: 10.7536/PC190201 前一篇   后一篇

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用于细胞检测的微电极传感器设计及传感分析

夏也1,2,4, 苏喜1,2,4, 陈李1,3,4,5, 李顺波1,3,4,5,**(), 徐溢1,2,3,4,5,**()   

  1. 1. 重庆大学新型微纳器件与系统技术重点学科实验室 重庆 400030
    2. 重庆大学化学化工学院 重庆 400030
    3. 重庆大学光电技术与系统教育部重点实验室 重庆 400030
    4. 重庆大学微纳系统与新材料国际联合研发中心 重庆 400030
    5. 重庆大学光电工程学院 重庆 400030
  • 收稿日期:2019-02-01 出版日期:2019-08-15 发布日期:2019-04-12
  • 通讯作者: 李顺波, 徐溢
  • 基金资助:
    国家自然科学基金项目(61971074); 国家自然科学基金项目(61904021); 重庆市人工智能技术创新重大主题专项项目(CSTC2017RGZN-ZDYFX0019); 重庆市技术创新与应用示范重大主题专项项目(CSTC2018JSZX-CYZTZX0216); 中央高校基本科研业务费专项高校基金(2019CDYGYB003)
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Design and Application of Electrochemical Sensor in Cell Detection

Ye Xia1,2,4, Xi Su1,2,4, Li Chen1,3,4,5, Shunbo Li1,3,4,5,**(), Yi Xu1,2,3,4,5,**()   

  1. 1. Key Disciplines Laboratory of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400030, China
    2. School of Chemistry and Chemical Engineering, Chongqing University, Chongqing 400030, China
    3. Key Laboratory for Optoelectronic Technology & System of Ministry of Education, Chongqing University, Chongqing 400030, China
    4. International R & D Center of Micro-Nano Systems and New Materials Technology, Chongqing University, Chongqing 400030, China
    5. School of Optoelectronics Engineering, Chongqing University, Chongqing 400030, China
  • Received:2019-02-01 Online:2019-08-15 Published:2019-04-12
  • Contact: Shunbo Li, Yi Xu
  • About author:
    ** E-mail: (Yi Xu)
    (Shunbo Li)
  • Supported by:
    National Natural Science Foundation of China(61971074); National Natural Science Foundation of China(61904021); Chongqing Arpngicial Intelligence Technology Innovation Major Special Project(CSTC2017RGZN-ZDYFX0019); Major Project of Technological Innovation and Application Demonstration of Chongqing(CSTC2018JSZX-CYZTZX0216); Special Funds for Basic Scienpngic Research in Central Universities(2019CDYGYB003)

本文针对肿瘤细胞的活性检测、神经细胞的神经递质检测与巨噬细胞等的氧化损伤检测等细胞检测中的核心问题,简要介绍电化学生化传感器和传感方法在细胞检测领域的应用和发展,重点对不同微电极结构的电化学传感器的设计制作、细胞检测方法及应用进展进行了综述。电化学生化传感器从单一检测电极向集成多功能和阵列式电极发展,从单个电极传感检测模式向芯片集成微电极式传感系统发展,而在其生物相容性、检测限和检测效率等方面尚需进一步提升和拓展。基于微机电系统(MEMS)技术制作的微电极研制,电极表面的多种化学和生物修饰的敏感膜研究,从硅基到聚合物柔性基底电极的材料拓展,小体积、植入式、可穿戴式的电化学生化传感器研制等是目前发展的方向,其在临床检验、精准医疗、运动健康监测、老年健康服务等诸多领域中显示出巨大的应用前景。

关键词： 细胞检测, 微电极, 微机电系统技术(MEMS技术), 活性氧, 柔性电极

The application and development of electrochemical sensors and sensing methods in the field of cell detection are introduced briefly, aiming at the core problems in cell detection, such as detection of tumor cell activity, neurotransmitters in nerve cells and oxidative damage in macrophages. The design, fabrication, detection and application of electrochemical sensors with different microelectrode structures are discussed in details. It is shown that electrochemical sensors have developed from single detection electrode to integrated multi-functional and array electrode, and from single electrode sensing detection mode to chip integrated microelectrode sensing system. However, its biocompatibility, detection limit and detection efficiency need to be further improved and expanded. The development of microelectrode based on MEMS(Micro-electro-mechanical system)technology, the research of various chemical and biological modified sensitive film on the electrode surface, the material expansion from silicon-based to polymer flexible base electrode, and the development of small volume, implantable and wearable electrochemical and biochemical sensors are the current development directions. It has also illustrated great application prospects in clinical examination, precision medicine, sports health monitoring, elderly health services and many other fields.

Key words: cell detection, microelectrodes, micro-electro-mechanical system technique, reactive oxygen, flexible electrode
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图1 Clark电极的发展:(A)传统Clark型电化学传感器校准示意图;(B)全固态微型溶解氧传感器结构示意图[18,19]
Fig. 1 Development of Clark sensors.(A)Calibration diagram of traditional Clark electrochemical sensor;(B)All solid state dissolved oxygen sensor[18,19]
图2 过氧化氢检测阵列芯片传感器实物图[27]
Fig. 2 Sensor diagram of hydrogen peroxide detection array chip[27]
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