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化学进展 2015, Vol. 27 Issue (9): 1240-1250 DOI: 10.7536/PC150132 前一篇   后一篇

• 综述与评论 •

新型纳米荧光探针在微流控细菌芯片检测中的应用

蒋艳1,3, 徐溢1,2,3*, 王人杰1,3, 苏喜1,3, 董春燕1   

  1. 1. 重庆大学化学化工学院 重庆 400030;
    2. 重庆大学新型微纳器件与系统技术国防重点学科实验室 重庆 400030;
    3. 重庆大学微纳系统及新材料技术国际研发中心 重庆 400030
  • 收稿日期:2015-01-01 修回日期:2015-05-01 出版日期:2015-09-15 发布日期:2015-06-24
  • 通讯作者: 徐溢 E-mail:xuyibbd@sina.com
  • 基金资助:
    国家自然科学基金项目(No.21375156),国家高技术研究发展计划(863)项目(No. 2015AA021104),重庆市科技攻关项目(CSCT, 2012gg B10001)和重点大学基础研究项目(No.106112015CDJZR225512)资助
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导出 被引次数 ( 6 | 3 )

Application of Novel Nano Fluorescent Probes for Bacteria Detection on the Microchip

Jiang Yan1,3, Xu Yi1,2,3*, Wang Renjie1,3, Su Xi1,3, Dong Chunyan1   

  1. 1. Chemistry and Chemical Engineering College, Chongqing University, Chongqing 400030, China;
    2. Defense Key Disciplines Lab of Novel Micro-Nano Devices and System Technology, Chongqing University, Chongqing 400030, China;
    3. International R&D Center of Micro-Nano Systems and New Materials Technology, Chongqing University, Chongqing 400030, China
  • Received:2015-01-01 Revised:2015-05-01 Online:2015-09-15 Published:2015-06-24
  • Supported by:
    supported by the National Natural Science Foundation of China (No.21375156), the National High Technology Research and Development Program of China (No.2015AA021104), the Research Project of New Detection Technology for Toxic Substances and Illegal Additive in Food, Drug (CSTC, 2012gg B1001), and the Fundamental Research Funds for the Central Universities(No.106112015CDJZR225512).
微流控芯片分析技术可以集成不同的生物化学分析功能单元,广泛应用于生化分析领域,在细菌检测方面具有传统检测方法不可比拟的优越性。近来年,在微流控细菌芯片中引入高荧光强度、低背景荧光干扰和高选择性的纳米荧光探针为实现细菌高效检测分析提供了新的研究途径和技术手段。本文通过对细菌检测中的几类新型荧光标记探针的介绍和比较,分析其荧光效应和应用特点,尤其是在细菌检测中的应用特性,重点综述了新型高效的纳米荧光探针与微流控细菌芯片分析方法和技术结合,实现微尺度空间和荧光检测模式下的细菌高效检测。
关键词： 纳米荧光探针, 微流控芯片, 细菌检测
Microfluidic system integrated with different functional units is widely employed in biochemical analysis and shows great potentials compared with conventional approaches for bacteria detection. Recently, novel nano fluorescent probes with improved fluorescent properties, low background and enhanced selectivity provide an efficient way for pathogenic bacteria quantitation, especially when they are combined with miniaturized platform or microfluidic device. Several novel nano fluorescent probes for bacteria detection are introduced and discussed in this review. Moreover, special attention has been paid to the recent research about combination of these approaches in bacteria analysis using variety of nano fluorescent probes on the microfluidic chip. It is also focused that the development of effective bacteria detection under the micro scale space with fluorescent monitoring mode.

Contents
1 Introduction
2 The technology of bacteria detection on microfluidic chips
3 New nano-fluorescent probes for bacteria labeled and detection
4 Detection of bacteria by nano-fluorescent probes on microfluidic chip
4.1 Detection of bacteria with nano-fluorescent probes
4.2 Detection of bacteria with nano-fluorescent probes and aptamers
4.3 Detection of bacteria with nano-fluorescent probes and immunoassay
5 Conclusion and perspective

Key words: nano fluorescent probes, microfluidic chip, bacteria detection

中图分类号: 

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