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化学进展 2014, Vol. 26 Issue (07): 1190-1201 DOI: 10.7536/PC140150 前一篇   后一篇

• 综述与评论 •

聚合物晶胶的制备、性能及生物医学应用

刘春桃, 童国权, 陈朝珠, 谭子芳, 全昌云, 张超*   

  1. 中山大学工学院 广州 510006
  • 收稿日期:2014-01-01 修回日期:2014-03-01 出版日期:2014-07-15 发布日期:2014-05-22
  • 通讯作者: 张超 E-mail:zhchao9@mail.sysu.edu.cn
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Polymeric Cryogel：Preparation, Properties and Biomedical Applications

Liu Chuntao, Tong Guoquan, Chen Chaozhu, Tan Zifang, Quan Changyun, Zhang Chao*   

  1. School of Engineering, Sun Yat-sen University, Guangzhou 510006, China
  • Received:2014-01-01 Revised:2014-03-01 Online:2014-07-15 Published:2014-05-22

利用低温凝胶化技术制备的聚合物晶胶，具有贯通多孔结构，因其化学/机械稳定性，可用于生物微粒（质粒、病毒、细胞器）和细胞的分离、生物分子和细胞的固定化载体以及组织工程三维支架等领域。本文详细介绍了聚合物晶胶的制备条件（如单体浓度、冷冻过程、引发剂浓度、溶剂等）与其结构、性能的关系；同时，对聚合物晶胶功能化改性及其在生物物质的色谱分离和生物医学领域的应用进行了总结与展望。

关键词： 聚合物晶胶, 低温凝胶化, 生物医学

Polymeric cryogel with interconnected pore structure can be prepared using the cryogelation technique and have been paid intensive attention in the fields of both academia and industry. Because of its high porosity, permeability, and chemical/mechanical stability, polymeric cryogel has been widely used as stationary phase in chromatography separation of particulate-containing fluids and cells, carriers for immobilization of cell/biological particulates, and three-dimensional scaffolds for tissue engineering. This paper introduces in detail the preparation of polymeric cryogel and relationship between its structural parameters and preparation process, such as concentration of monomer/precursor/initiator, freezing process, and solvent. The functionalization of cryogels as well as its biomedical applications are also summarized.

Contents
1 Introduction
1.1 Preparation and structure of polymeric cryogels
1.2 Pore structure and the preparation process
1.3 Structure characterization of cryogels
2 Functionalization of polymeric cryogels
2.1 Copolymerization of functional monomers
2.2 Surface coupling of functional groups
2.3 Surface graft polymerization
2.4 Double crosslinking
2.5 Composite cryogels
3 Application of polymeric cryogels
3.1 Stationary phase in chromatography separation of cell/biological particulate
3.2 Carrier for immobilization of cell/biological particulate
3.3 Scaffold for tissue engineering
4 Conclusion and outlook

Key words: polymeric cryogel, cryogelation, biomedical

中图分类号: 

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[1] Hjertén S, Liao J L, Zhang R. J. Chromatogr. A, 1989, 473: 273.
[2] Nam Y S, Park T G. J. Biomed. Mater. Res., 1999, 47: 8.
[3] Wood C D, Cooper A I. Macromolecules, 2001, 34: 5.
[4] Kabiri K, Omidian H, Zohuriaan-Mehr M J. Polym. Int., 2003, 52: 1158.
[5] Hentze H P, Antonietti M. Rev. Mol. Biotechnol., 2002, 90(1): 27.
[6] Lozinsky V I, Galaev I Y, Plieva F M, Savina I N, Jungvid H, Mattiasson B. Trends Biotechnol., 2003, 21: 445.
[7] Plieva F M, Galaev I Y, Mattiasson B. J. Sep. Sci., 2007, 30: 1657.
[8] Plieva F M, Karlsson M, Aguilar M R, Gomez D, Mikhalovsky S, Galaev I Y. Soft Matter, 2005, 1: 303.
[9] Plieva F M, Savina I N, Deraz S, Andersson J, Galaev I Y, MattiassonB. J. Chromatogr. B, 2004, 807(1): 129.
[10] Plieva F M, Xiao H T, Galaev I Y, Bergensthl B, Mattiasson B. J. Mater. Chem., 2006, 16: 4065.
[11] Hwang Y S, Zhang C, Varghese S. J. Mater. Chem., 2010, 20: 345.
[12] Tang S Z, Shen S C, Yun J X, Yao K J. J. Chem. Eng. Chin. Univ., 2010, 24(6): 974.
[13] 王良华(Wang L H). 浙江大学博士论文(Doctoral Dissertation of Zhejiang University),2008
[14] 陈芳(Chen F). 浙江工业大学硕士论文(Master Dissertation of Zhejiang University of Technology), 2008.
[15] Bencherif S A, Sands R W, Bhatta D, Arany P, Verbeke C S, Edwards D A, Mooney D J. Proc. Natl. Acad. Sci. USA, 2012, 109(48): 19590.
[16] Dainiak M B, Kumar A, Galaev I Y, Mattiasson B. Proc. Natl. Acad. Sci. USA, 2006, 103(4): 849.
[17] Plieva F M, Bober B, Dainiak M, Galaev I Y, Mattiasson B. J. Mol. Recognit., 2006, 19: 305.
[18] Ozmen M M, Okay O. Polymer, 2005, 46(19): 8119.
[19] Dainiak M B, Savina I N, Musolino I, Kumar A, Mattiasson B, Galaev I Y. Biotechnol. Prog., 2008, 24(6): 1373.
[20] Arvidssona P, Plieva F M, Lozinskyb V I, Galaeva I Y, Mattiasson B. J. Chromatogr. A, 2003, 986: 275.
[21] Kumar A, Plieva F M, Galaeva I Y, Mattiasson B. J. Immunol. Methods., 2003, 283: 185.
[22] Lysogorskaya E N, Roslyakova T V, Belyaeva A V, Lozinskii V I, Filippova I Y. Appl. Biochem. Microbiol., 2008, 44(3): 241.
[23] Savina I N, Galaev I Y, Mattiasson B. J. Chromatogr. A, 2005, 1092(2): 199.
[24] Savina I N, Galaev I Y, Mattiasson B. J. Mol. Recognit., 2006, 19: 313.
[25] Savina I N, Galaev I Y, Mattiasson B. Polymer, 2005, 46: 9596.
[26] Plieva F M, Ekstrom P, Galaev I Y, Mattiasson B. Soft Matter, 2008, 4: 2418.
[27] Zhao Q, Sun J Z, Wu X F, Lin Y T. Soft Matter, 2011, 7(9): 4284.
[28] Yao K J, Yun J X, Shen S C, Wang L H, He X J, Yu X M. J. Chromatogr. A, 2006, 1109: 103.
[29] Noir M L, Plieva F M, Hey T, Guieysse B, Mattiasson B. J. Chromatogr. A, 2007, 1154: 158.
[30] Hajizadeh S, Kirsebom H, Mattiasson B. Soft Matter, 2010, 6: 5562.
[31] Kueseng P, Thammakhet C, Thavarungkul P, Kanatharana P. Microchem. J., 2010, 96: 317.
[32] Xu P P, Yao Y C, Shen S C, Yun J X, Yao K J. Chin. J. Nucl. Phys., 2010, 18(4): 667.
[33] De Cock L J, De Wever O, Vlierberghe S, Vanderleyden E, Dubruel P, De Vos F, Vervaet C, Remon J P, De Geest B G. Soft Matter, 2012, 8(4): 1146.
[34] Arvidsson P, Plieva F M, Savina I N, Lozinsky V I, Fexby S, Bulow L, Galaev I Y, Mattiasson B. J. Chromatogr. A, 2002, 977: 27.
[35] Arvidsson P, Plieva F M, Lozinsky V I, Mattiasson B. J. Chromatogr. A, 2003, 986:275.
[36] Dainiak M B, Plieva F M, Galaev I Y, Hatti-kaul R, Mattiasson B. Biotechnol. Prog., 2005, 21: 644.
[37] Babac C, Yavuz H, Galaev I Y, Piskin E, Denizli A. React. Funct. Polym., 2006, 66(11): 1263.
[38] Yan L D, Shen S C, Yun J X, Yao K J. Chin. J. Nucl. Phys., 2011, 5: 876.
[39] Zhan X Y, Lu D P, Lin D Q, Yao S J. J. Appl. Polym. Sci., 2013, 130(5): 3082.
[40] Shen S C, Wang L Y, Sun Z T, Li M F, Liu C Z, Tian B, Yun J X, Hua Y J. Chin. J. Nucl. Phys., 2013, 21(6): 663.
[41] 沈绍传(Shen S C). 浙江大学博士论文( Doctoral Dissertation of Zhejiang University), 2011.
[42] Yu X M, Zhao P, Zhang W B, Zhang Y K, Zhang L H. Prog. Modern Biomed., 2013, 13(6): 1015.
[43] 何兴娇(He X J). 浙江工业大学硕士论文(Master Dissertation of Zhejiang University of Technology), 2007.
[44] Guo Y T, Shen S C, Yun J X, Yao K J. Chin. J. Biotech., 2012, 28(8): 995.
[45] Yun J X, Dafoe J T, Peterson E, Xu L H, Yao S J, Daugulis A J. J. Chromatogr. A, 2013, 1284: 148.
[46] Wang L H, Chen F, Yun J X, Yao K J, Yao S J. J. Chem. Eng. Chin.Univ., 2009, 23(3): 480.
[47] Yun J X, Dafoe J T, Peterson E, Xu L H, Yao S J, Daugulis A J. J. Chromatogr. A, 2013, 1284: 148.
[48] Dainiak M B, Kumar A, Plieva F M, Mattiasson B. J. Chromatogr. A, 2004, 1045: 93.
[49] Noppe W, Plieva F M, Galaev I Y, Mattiasson B. J. Chromatogr. A, 2006, 1101: 79.
[50] Plieva F M, Karlsson, Mattiasson B. J. Appl. Polym. Sci., 2006, 100(2): 1057.
[51] Bacheva A V, Plieva F M, Lysogorskaya E N, FilippovaI Y, Lozinsky V I. Bioorg. Med. Chem. Lett., 2001, 11: 1005.
[52] Plieva F M, Kochetkov K A, Singh I, Parmar V S, Belokon Y N, Lozinsky V I. Biotechnol. Lett., 2000, 22: 551.
[53] Bereli N, Anda M, Baydemir G, Say R, Filippova I Y, Galaev I Y, Denizli A. J. Chromatogr. A, 2008, 1190: 18.
[54] Baydemir G, Bereli N, Anda M, Say R, Galaev I Y, Denizli A. Colloid. Surface. B, 2009, 68: 33.
[55] Derazshamshir A, Baydemir G, Andac M, Say R, Galaev I Y, Denizli A. Macromol. Chem. Phys., 2010, 211: 657.
[56] Deng Y P, Zhang G H, Xu L H, Shen S C, Yun J X, Yao K J. J. Chem. Eng. Chin. Univ., 2013, 21(1): 119.
[57] Chen Z Y, Xu L, Liang Y, Wang J B, Zhao M P, Li Y Z. J. Chromatogr. A, 2007, 1182: 128.
[58] Bloch K, LozinskyV I, Galaev I Y, Yavriyanz K, Vorobeychik M, Azarov D, Damshkaln L G, Mattiasson B, Vardi1 P. J. Biomed. Mater. Res., Part A, 2005, 75A(4): 802.
[59] Sun S J, Tang Y H, Fu Q, Zhao Y D. J. Sep. Sci., 2012, 35(7): 893.
[60] Sun S J, Tang Y H, Fu Q, Liu X, Guo Y A, Zhao Y D, Chang C. Int. J Biol. Macromol., 2012, 50(4): 1002.
[61] Kathuria N, Tripathi A, KumarA. Acta. Metall., 2009, 5: 406.
[62] Arrua R D, Haddad P R, Hilder E F. J. Chromatogr. A, 2013, 1311: 121.
[63] 张凯(Zhang K). 中国地质大学硕士论文(Master Dissertation of China University of Geosciences), 2012.
[64] Kumakura M. Polym. Adv. Technol., 2001, 12: 415.
[65] Sutani K, Kaetsu I, Uchida K. Radiat. Phys. Chem., 2001, 61: 49.
[66] Hayashi T, Hyon S H, Cha W I, Ikada Y. J. Appl. Polym. Sci., 1993, 49: 2121.
[67] Belokon Y N, Kochetkov K A, Plieva F M. Appl. Biochem. Biotechnol., 2000, 88: 97.
[68] Filippova I Y, Bacheva A V, Baibak O V, Plieva F M, Lysogorskaya E N, Oksenoit E S, Lozinsky V I. Russ. Chem. Bull., 2001, 50: 1896.
[69] Lusta K A, Starostina N G, Gorkina N B, Fikhte B A, Lozinski V I. Prikl. Biokhim. Mikrobiol., 1988, 24(4): 504.
[70] Kumar A, Caballero A R, Plieva F M, Galaev I Y, Nandakumar K S, Kamihira M, Holmdahl R, Orfao A, Mattiasson B. J. Mol. Recognit., 2005, 18: 84.
[71] Lusta K A, Chung K, Sul W, Park H S, Shin D. Process. Biochem., 2000, 35: 1177-1182
[72] Scardi V. Methods Enzymol., 1987, 135B: 293.
[73] Kirsebom H, Aguilar M R, Roman J S, Fernandez M, Prieto M A, Bondar B. J. Bioact. Compat. Pol, 2007, 22(6): 621.
[74] Jurga M, Dainiak M B, Sarnowska A, Jablonska A, Tripathi A, Plieva F M, Savina I N, Strojek L, Jungvid H, Kumar A, Lukomska B, Janik K D, Forraz N, McGuckin C P. Biomaterials, 2011, 32(13): 3423.
[75] Tripathi A, Kathuria N, Kumar A. J. Biomed. Mater. Res. Part A, 2009, 90A(3): 680.
[76] Tripathi A, Kumar A. Macromol. Biosci., 2011, 11(1): 22.
[77] Tripathi A, Vishnoi T, Singh D, Kumar A. Macromol. Biosci., 2013, 13: 838.
[78] Liu Y, Vrana N E, Cahil P A, McGuinness G B. J. Biomed. Mater. Res.B, 2009, 90B(2): 492.
[79] Mathews D T, Birney Y A, Cahill P A, McGuinness G B. J. Biomed. Mater. Res. B, 2008, 84B(2): 531.
[80] Bajpai A K, Saini R. J. Mater. Sci. Mater. Med., 2009, 20(10): 2063.
[81] Vrana N E, Cahill P A, McGuinness G B. J. Biomed. Mater. Res. A, 2010, 94A(4): 1080.
[82] Vrana N E, Matsumura K, Hyon S H, Geever L M, Kennedy J E, Lyons J G, Higginbotham C L, Cahill P A, McGuinness G B. J. Tissue. Eng. Regen. Med., 2012, 6(4): 280.
[83] Bolgen N, Vargel I, Korkusuz P. J. Biomed. Mater. Res. A, 2009, 91A(1): 60.
[84] Singh D, Nayak V, Kumar A. Int. J. Biol. Sci., 2010, 6(4): 371.
[85] Savina I N, Dainiak M, Jungvid H, Mikhalovcky S V, Galaev L Y. J. Biomater. Sci., Polym. Ed., 2009, 20(12): 1781.
[86] Blgen N, Yang Y, Korkusuz P, Güzel E, Haj A J, Pi?kin E. J. Tissue. Eng.Regen.Med., 2011, 5(10): 770.
[87] Singh D, Tripathi A, Nayak V, Kumar A. J. Biomater. Sci., Polym. Ed., 2011, 22(13): 1733.
[88] Phadke A, Hwang Y S, Kim S H, Kim S H, Yamaguchi T, Masuda K, Varghese S. Eur. Cells. Mater., 2013, 25: 114.
[89] Zhan X Y, Lu D P, Lin D Q, Yao S J. J. Appl. Polym. Sci., 2013, 12(5): 3082.
[90] Mu C D, Liu F, Cheng Q S, Li H L, Wu B, Zhang G Z, Lin W. Macromol. Mater. Eng., 2010, 295(2): 100.
[91] Jiang S, Liu S, Feng W H. J. Mech. Behav. Biomed., 2011, 4(7): 1228.
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